Rabbit anti-Human VPS33A Polyclonal Antibody | anti-VPS33A antibody

VPS33A Antibody

Cat. Num. AAA7050935

• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: VPS33A; Polyclonal Antibody; VPS33A Antibody; Vacuolar protein sorting-associated protein 33A; hVPS33A; anti-VPS33A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 596

• Applicable Applications for anti-VPS33A antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Vacuolar protein sorting-associated protein 33A protein (1-300AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7050935 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of U251 cells using MBS7050935 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-VPS33A antibody

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed: 23351085, PubMed: 24554770, PubMed: 25266290, PubMed: 25783203). Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS16 but not VIPAS39 (PubMed: 25783203). The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG (PubMed: 24554770).

NCBI and Uniprot Product Information

• NCBI GI #: 18105056
• NCBI GeneID: 65082
• NCBI Accession #: NP_075067.2
• NCBI GenBank Nucleotide #: NM_022916.4
• UniProt Accession #: Q96AX1; Q547V4; Q9H5Q0
• Molecular Weight: 67,611 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 33A
• NCBI Official Synonym Full Names: VPS33A, CORVET/HOPS core subunit
• NCBI Official Symbol: VPS33A
• NCBI Protein Information: vacuolar protein sorting-associated protein 33A
• UniProt Protein Name: Vacuolar protein sorting-associated protein 33A
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS33A
• UniProt Synonym Gene Names: hVPS33A
• UniProt Entry Name: VP33A_HUMAN

NCBI Description

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and it encodes a protein similar to the yeast class C Vps33 protein. The mammalian class C VPS proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS33A: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Belongs to the STXBP/unc-18/SEC1 family.

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: early endosome; late endosome; lysosomal membrane; perinuclear region of cytoplasm

Molecular Function: protein binding

Biological Process: endosome to lysosome transport; lysosome localization; melanosome localization; platelet formation; regulation of pigmentation during development; vesicle-mediated transport