Rabbit VP16 Polyclonal Antibody | anti-VP16 antibody

VP16 tag Polyclonal Antibody

Cat. Num. AAA3015403

• Reactivity: Human herpesvirus 1
• Applications: Western Blot
• Purity: affinity purified by Protein A
• Synonyms: VP16; Polyclonal Antibody; VP16 tag Polyclonal Antibody; VP16_HHV1F;UL48; Tegument protein VP16; Alpha trans-inducing protein; Alpha-TIF; ICP25; Vmw65; anti-VP16 antibody

• Host: Rabbit
• Reactivity: Human herpesvirus 1
• Clonality: Polyclonal
• Specificity: VP16 tag Polyclonal Antibody detects endogenous levels of VP16 tag protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 122% Glycerol.

• Applicable Applications for anti-VP16 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-2000
• Immunogen: KLH conjugated synthetic peptide derived from HSV-1 VP16:391-490/490aa
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -41 degree C long term. Avoid freeze-thaw cycles.

Testing Data

(Primary: Anti-VP16 tag at 1/1000 dilution)

Related Product Information for anti-VP16 antibody

HSV evades the immune system through interference with MHC class I antigen presentation on the cell surface, by blocking TAP or the transporter associated with antigen processing induced by the secretion of ICP-47 by HSV. In the host cell, TAP transports digested viral antigen epitope peptides from the cytosol to the endoplasmic reticulum, allowing these epitopes to be combined with MHC class I molecules and presented on the surface of the cell. Viral epitope presentation with MHC class I is a requirement for activation of cytotoxic T-lymphocytes (CTLs), the major effectors of the cell-mediated immune response against virally-infected cells. ICP-47 prevents initiation of a CTL-response against HSV, allowing the virus to survive for a protracted period in the host.

NCBI and Uniprot Product Information

• NCBI GI #: 259016402
• UniProt Accession #: P04486
• Molecular Weight: 70.3 kDa
• NCBI Official Full Name: Tegument protein VP16
• UniProt Protein Name: Tegument protein VP16
• UniProt Gene Name: UL48
• UniProt Entry Name: VP16_HHV1F

Uniprot Description

Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection ().