Rabbit TAP1 Polyclonal Antibody | anti-TAP1 antibody

TAP1 Antibody

Cat. Num. AAA9612494

• Gene Names: TAP1; APT1; PSF1; ABC17; ABCB2; PSF-1; RING4; TAP1N; D6S114E; TAP1*0102N
• Reactivity: Human, Mouse, Monkey
• Applications: ELISA
• Purity: Peptide affinity purification
• Synonyms: TAP1; Polyclonal Antibody; TAP1 Antibody; ABC 17; ABC transporter MHC 1; ABC17; ABCB 2; ABCB2; Antigen peptide transporter 1; APT 1; APT1; ATP binding cassette sub family B (MDR/TAP) member 2; ATP binding cassette sub family B member 2; ATP binding cassette transporter; ATP-binding cassette sub-family B member 2; D6S114E; FLJ26666; FLJ41500; Peptide supply factor 1; Peptide transporter involved in antigen processing 1; Peptide transporter PSF 1; Peptide transporter PSF1; Peptide transporter TAP 1; Peptide transporter TAP1; PSF 1; PSF-1; PSF1; Really interesting new gene 4 protein; RING 4; RING4; TAP 1; TAP1*0102N; TAP1_HUMAN; TAP1N; Transporter 1 ATP binding cassette sub family B (MDR/TAP); Transporter 1 ATP binding cassette sub family B; Transporter associated with antigen processing; Transporter ATP binding cassette major histocompatibility complex 1; Y3; anti-TAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TAP1 Antibody detects endogenous levels of total TAP1.
• Purity/Purification: Peptide affinity purification
• Form/Format: IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 808

• Applicable Applications for anti-TAP1 antibody: ELISA (EIA)
• Application Notes: IF: 1:100-1:500; ICC: 1:100-1:500; WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000; ; IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Immunogen: A synthesized peptide derived from human TAP1, corresponding to a region within N-terminal amino acids.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using TAP1 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from various samples, using TAP1 Antibody.Lane 1: COS, treated with blocking peptide;Lane 2: COS;Lane 3: Mouse brain.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612494 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612494 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Related Product Information for anti-TAP1 antibody

Function: Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.

NCBI and Uniprot Product Information

• NCBI GI #: 9665248
• NCBI GeneID: 6890
• NCBI Accession #: NP_000584.2
• NCBI GenBank Nucleotide #: NP_000584.2
• UniProt Accession #: Q03518
• Molecular Weight: Observed MW: 87kD.
• NCBI Official Full Name: antigen peptide transporter 1 isoform 1
• NCBI Official Synonym Full Names: transporter 1, ATP binding cassette subfamily B member
• NCBI Official Symbol: TAP1
• NCBI Official Synonym Symbols: APT1; PSF1; ABC17; ABCB2; PSF-1; RING4; TAP1N; D6S114E; TAP1*0102N
• NCBI Protein Information: antigen peptide transporter 1
• UniProt Protein Name: Antigen peptide transporter 1
• Protein Family: Protein
• UniProt Gene Name: TAP1
• UniProt Synonym Gene Names: ABCB2; PSF1; RING4; Y3; APT1; PSF-1
• UniProt Entry Name: TAP1_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]

Uniprot Description

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.