Rabbit NFX1 Polyclonal Antibody | anti-NFX1 antibody

NFX1 Polyclonal Antibody

Cat. Num. AAA3005564

• Gene Names: NFX1; NFX2; Tex42; TEG-42
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: affinity purified by Protein A
• Synonyms: NFX1; Polyclonal Antibody; NFX1 Polyclonal Antibody; NFX2; MGC20369; NFX 1; Nuclear transcription factor X box binding 1; Nuclear transcription factor X box binding protein 1; Transcriptional repressor NF X1; NFX1_HUMAN; anti-NFX1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: NFX1 Polyclonal Antibody detects endogenous levels of NFX1 protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-NFX1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-2000
• Immunogen: KLH conjugated synthetic peptide derived from human NFX1:881-980/1120
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Testing Data

(Primary: Anti-NFX1 at 1/1000 dilution)

Testing Data

(Primary: Anti-NFX1 at 1/1000 dilution)

Related Product Information for anti-NFX1 antibody

NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. It may play a role in the inflammatory response, regulating its duration by limiting the period in which class II MHC molecules are induced by IFN gamma. The RING type zinc finger domain interacts with an ubiquitin conjugating enzyme (E2) and facilitates ubiquitination.

NCBI and Uniprot Product Information

• NCBI GI #: 21361137
• NCBI GeneID: 4799
• NCBI Accession #: NP_002495.2
• NCBI GenBank Nucleotide #: NM_002504.5
• UniProt Accession #: Q12986; Q5VXW6; Q96EL5; Q9BXI1; A8K6H8
• Molecular Weight: 92,679 Da
• NCBI Official Full Name: transcriptional repressor NF-X1 isoform 1
• NCBI Official Synonym Full Names: nuclear transcription factor, X-box binding 1
• NCBI Official Symbol: NFX1
• NCBI Official Synonym Symbols: NFX2; Tex42; TEG-42
• NCBI Protein Information: transcriptional repressor NF-X1
• UniProt Protein Name: Transcriptional repressor NF-X1
• Protein Family: NFX1-type zinc finger-containing protein
• UniProt Gene Name: NFX1
• UniProt Synonym Gene Names: NFX2

NCBI Description

MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

NFX1: Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination. Belongs to the NFX1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ligase; Transcription factor; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: cytosol; nucleolus; nucleus; plasma membrane

Molecular Function: DNA binding transcription factor activity; ligase activity; RNA binding; zinc ion binding

Biological Process: inflammatory response; negative regulation of MHC class II biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; viral process