Rabbit anti-Mouse CD8 Polyclonal Antibody | anti-CD8 antibody

CD8 polyclonal antibody

Cat. Num. AAA3013853

• Gene Names: Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
• Reactivity: Mouse
• Applications: Western Blot
• Purity: affinity purified by Protein A
• Synonyms: CD8; Polyclonal Antibody; CD8 polyclonal antibody; CD8 antigen; alpha polypeptide (p32); CD8a; CD8a antigen; CD8a molecule; CD8A_MOUSE; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen; anti-CD8 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: CD8 polyclonal Antibody detects endogenous levels of CD8 protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-CD8 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-2000
• Immunogen: KLH conjugated synthetic peptide derived from mouse CD8:51-150/247
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Testing Data

(Blood (Mouse) Lysate at 40 ug Anti- CD8 at 1/1000 dilution)

Related Product Information for anti-CD8 antibody

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011].

NCBI and Uniprot Product Information

• NCBI GI #: 124487437
• NCBI GeneID: 12525
• NCBI Accession #: NP_001074579.1
• NCBI GenBank Nucleotide #: NM_001081110.2
• UniProt Accession #: P01731
• Molecular Weight: 27456 MW
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain isoform 1
• NCBI Official Synonym Full Names: CD8 antigen, alpha chain
• NCBI Official Symbol: Cd8a
• NCBI Official Synonym Symbols: Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: CD81 antigen
• UniProt Gene Name: Cd8a
• UniProt Synonym Gene Names: Lyt-2; Lyt2

Uniprot Description

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.