Western Blot (WB)
((1ug/ml) staining of Human Placenta (A), Human Uterus (B), Human Adrenal Gland (C) and Human Pancreas (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat VMAT2/SLC18A2 Polyclonal Antibody | anti-SLC18A2 antibody
Goat anti-VMAT2/SLC18A2 Antibody
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Western blot: Approx 55-58kDa band observed in Human Adrenal Gland and approx. 50kDa in Mouse Brain lysates (calculated MW of 55.7kDa according to Human NP_003045.2 and Mouse NP_766111.1). Recommended concentration 1-3ug/ml. Primary incubation 1 hour at room temperature.
Western Blot (WB)
((1ug/ml) staining of Human Placenta (A), Human Uterus (B), Human Adrenal Gland (C) and Human Pancreas (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Placenta (A), Human Uterus (B), Human Adrenal Gland (C) and Human Pancreas (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
(MBS421308 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.)
Immunohistochemistry (IHC)
(MBS421308 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.)
Immunohistochemistry (IHC)
(MBS421308 (2.5ug/ml) staining of paraffin embedded Human Adrenal Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.This data is from a previous batch, not on sale.)
Immunohistochemistry (IHC)
(MBS421308 (2.5ug/ml) staining of paraffin embedded Human Adrenal Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.This data is from a previous batch, not on sale.)
Immunohistochemistry (IHC)
(MBS421308 Negative Control showing staining of paraffin embedded Human Adrenal Gland, with no primary antibody. This data is from a previous batch, not on sale.)
Immunohistochemistry (IHC)
(MBS421308 Negative Control showing staining of paraffin embedded Human Adrenal Gland, with no primary antibody. This data is from a previous batch, not on sale.)
NCBI and Uniprot Product Information
NCBI Description
The vesicular monoamine transporter acts to accumulate cytosolic monoamines into synaptic vesicles, using the proton gradient maintained across the synaptic vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine (summary by Peter et al., 1993 [PubMed 7905859]). See also SLC18A1 (MIM 193002).[supplied by OMIM, Jan 2011]
Uniprot Description
SLC18A2: Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis. Belongs to the major facilitator superfamily. Vesicular transporter family.
Protein type: Transporter; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 10q25
Cellular Component: cell soma; integral to plasma membrane; membrane; plasma membrane; synaptic vesicle; synaptic vesicle membrane; terminal button
Molecular Function: amine transmembrane transporter activity; drug binding; enzyme binding; heat shock protein binding; monoamine transmembrane transporter activity; serotonin transmembrane transporter activity
Biological Process: aging; dopamine transport; endocytic recycling; glucose homeostasis; insulin secretion; locomotory behavior; monoamine transport; negative regulation of neurotransmitter transport; neurotransmitter secretion; post-embryonic development; response to amphetamine; response to cocaine; response to corticosterone stimulus; response to herbicide; response to starvation; response to zinc ion; sequestering of neurotransmitter; serotonin transport; synaptic transmission; synaptic vesicle amine transport; transmembrane transport
Research Articles on SLC18A2
Similar Products
Product Notes
The SLC18A2 slc18a2 (Catalog #AAA421308) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-VMAT2/SLC18A2 Antibody reacts with Tested: Human, Mouse Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's VMAT2/SLC18A2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 55-58kDa band observed in Human Adrenal Gland and approx. 50kDa in Mouse Brain lysates (calculated MW of 55.7kDa according to Human NP_003045.2 and Mouse NP_766111.1). Recommended concentration 1-3ug/ml. Primary incubation 1 hour at room temperature. Researchers should empirically determine the suitability of the SLC18A2 slc18a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SYPIGEDEES ESD. It is sometimes possible for the material contained within the vial of "VMAT2/SLC18A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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