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Western Blot (WB) (Western Blot: Sample: Mouse Brain Tissue.)

Rabbit anti-Mouse Programmed Cell Death Protein 6 Interacting Protein Polyclonal Antibody | anti-PDCD6IP antibody

Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) Polyclonal Antibody

Gene Names
Pdcd6ip; Aip1; Alix; Eig2; C76364; AI480591; AW544830; mKIAA1375
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Programmed Cell Death Protein 6 Interacting Protein; Polyclonal Antibody; Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) Polyclonal Antibody; PDCD6-IP; AIP1; Alix; DRIP4; HP95; ALG-2 Interacting Protein X; anti-PDCD6IP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PDCD6IP. It has been selected for its ability to recognize PDCD6IP in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
751
Applicable Applications for anti-PDCD6IP antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Mouse
Organism Species
Mouse
Fragment
PDCD6IP (Met1~Lys239)
Quality Control
Content: The quality control contains recombinant PDCD6IP (Met1~Lys239) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2053103
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Brain Tissue.)

Western Blot (WB) (Western Blot: Sample: Mouse Brain Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant PDCD6IP, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant PDCD6IP, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Uterus Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Uterus Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Liver Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Pancreas Tissue)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
96,773 Da
NCBI Official Full Name
programmed cell death 6-interacting protein isoform 1
NCBI Official Synonym Full Names
programmed cell death 6 interacting protein
NCBI Official Symbol
Pdcd6ip
NCBI Official Synonym Symbols
Aip1; Alix; Eig2; C76364; AI480591; AW544830; mKIAA1375
NCBI Protein Information
programmed cell death 6-interacting protein
UniProt Protein Name
Programmed cell death 6-interacting protein
UniProt Gene Name
Pdcd6ip
UniProt Synonym Gene Names
Aip1; Alix

Uniprot Description

Alix: an adaptor protein that may regulate the function of receptor and cytoskeleton-associated tyrosine kinases. A class E vacuolar protein sorting (VPS) factor involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. May play a role in the regulation of both apoptosis and cell proliferation.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 9|9 F3

Cellular Component: actomyosin; bicellular tight junction; cell junction; cytoplasm; cytoskeleton; cytosol; endoplasmic reticulum exit site; extracellular exosome; extracellular region; Flemming body; focal adhesion; immunological synapse; membrane; myelin sheath

Molecular Function: calcium-dependent protein binding; protein binding; protein dimerization activity; protein homodimerization activity; proteinase activated receptor binding; SH3 domain binding

Biological Process: actomyosin contractile ring assembly; apoptosis; cell cycle; cell division; cell separation after cytokinesis; maintenance of epithelial cell polarity; mitotic cytokinesis; non-lytic virus budding; protein homooligomerization; protein transport; regulation of centrosome duplication; regulation of membrane permeability; transport

Research Articles on PDCD6IP

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Product Notes

The PDCD6IP pdcd6ip (Catalog #AAA2032700) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Programmed Cell Death Protein 6 Interacting Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PDCD6IP pdcd6ip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Programmed Cell Death Protein 6 Interacting Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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