Goat NOXA Polyclonal Antibody | anti-NOXA antibody

GOAT ANTI HUMAN NOXA

Cat. Num. AAA224213

• Gene Names: PMAIP1; APR; NOXA
• Applications: ELISA, Western Blot
• Synonyms: NOXA; Polyclonal Antibody; GOAT ANTI HUMAN NOXA; anti-NOXA antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises human Noxa, a mitochondrial localised protein and member of the BH3-only proapoptotic protein family, which is up-regulated by p53, IFNbeta, phorbol esters, dsRNA and viruses, and acts as promoter of caspase and apoptosis activation. ; ; Noxa is a transcriptional target of p53, p73 and BH3-only proteins, and competes with, and displaces, the proapoptotic proteins BAK1, and BIM, from the antiapoptotioc protein MCL1 (Bcl-2-related protein EAT/mcl1), thereby influencing apoptosis versus cell survival.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 54

• Applicable Applications for anti-NOXA antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 16kDa in human cerebellum cell lysates. Suitable loading control: Goat anti Human GAPDH; ELISA: Minimum Dilution: 1/16000; Western Blotting: Minimum Dilution: 0.03; Maximum Dilution: 0.1ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Immunogen: Synthetic peptide sequence C-TQLRRFGDKLNFRQK from the internal region of Noxa (NP_066950.1).; Buffer Solution
• Antiserum Preparation: Antiserum to human NOXA was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Human cerebellum cell lysate probed with Goat anti Human Noxa (MBS224213))

Related Product Information for anti-NOXA antibody

MBS224213 specifically recognises human Noxa, a mitochondrial localised protein and member of the BH3-only proapoptotic protein family, which is up-regulated by p53, IFNbeta, phorbol esters, dsRNA and viruses, and acts as promoter of caspase and apoptosis activation. Noxa is a transcriptional target of p53, p73 and BH3-only proteins, and competes with, and displaces, the proapoptotic proteins BAK1, and BIM, from the antiapoptotioc protein MCL1 (Bcl-2-related protein EAT/mcl1), thereby influencing apoptosis versus cell survival.

NCBI and Uniprot Product Information

• NCBI GI #: 10863923
• NCBI GeneID: 5366
• NCBI Accession #: NP_066950.1
• NCBI GenBank Nucleotide #: NM_021127.2
• UniProt Accession #: Q13794; Q8N589; B2R4T7
• Molecular Weight: 14,955 Da
• NCBI Official Full Name: phorbol-12-myristate-13-acetate-induced protein 1
• NCBI Official Synonym Full Names: phorbol-12-myristate-13-acetate-induced protein 1
• NCBI Official Symbol: PMAIP1
• NCBI Official Synonym Symbols: APR; NOXA
• NCBI Protein Information: phorbol-12-myristate-13-acetate-induced protein 1; PMA-induced protein 1; adult T cell leukemia-derived PMA-responsive; immediate-early-response protein APR; protein Noxa
• UniProt Protein Name: Phorbol-12-myristate-13-acetate-induced protein 1
• Protein Family: Superoxide-generating NADPH oxidase
• UniProt Gene Name: PMAIP1
• UniProt Synonym Gene Names: NOXA; PMA-induced protein 1
• UniProt Entry Name: APR_HUMAN

Uniprot Description

NOXA: Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1. Interacts with MCL1, BCL2A1 and BAX. Up-regulated by p53/TP53, phorbol esters, double- stranded RNA, IFNB1/IFN-beta and viruses. Highly expressed in adult T-cell leukemia cell line. Belongs to the PMAIP1 family.

Protein type: Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 18q21.32

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol; nucleus

Molecular Function: protein binding

Biological Process: proteasomal protein catabolic process; caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; apoptosis; response to dsRNA; positive regulation of caspase activity; cellular response to glucose starvation; positive regulation of protein oligomerization; negative regulation of fibroblast proliferation; positive regulation of neuron apoptosis; T cell homeostasis; regulation of mitochondrial membrane permeability; positive regulation of DNA damage response, signal transduction by p53 class mediator; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to X-ray; response to UV