Rabbit VLK Polyclonal Antibody | anti-Pkdcc antibody

VLK Antibody

Cat. Num. AAA150058

• Gene Names: Pkdcc; Vlk; MAd1; Adtk1; ESTM17; Sgk493; X83346; AI115348; AW548124
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: VLK Antibody is affinity chromatography purified via peptide column.
• Synonyms: VLK; Polyclonal Antibody; VLK Antibody; Vlk; MAd1; Adtk1; ESTM17; Sgk493; X83346; AI115348; AW548124; Protein kinase domain-containing protein; cytoplasmic; Protein kinase-like protein SgK493; protein kinase domain containing; anti-Pkdcc antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: VLK Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 492

• Applicable Applications for anti-Pkdcc antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: VLK antibody can be used for detection of VLK by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: VLK antibody was raised against a 15 amino acid synthetic peptide from near the center of human VLK.
• Buffer: VLK Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: VLK antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of VLK in human lung tissue lysate with VLK antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of VLK in rat lung tissue with VLK antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of VLK in rat lung tissue with VLK antibody at 5 μg/mL.)

Western Blot (WB)

(Western blot analysis of VLK expression in Jurkat cell lysate with VLK antibody at 0.5 μg/ml.)

Related Product Information for anti-Pkdcc antibody

VLK Antibody: VLK was identified as a novel protein kinase that was induced after the differentiation of cultured embryonic stem cells into mesendoderm. It has no homologs in invertebrates, but is highly conserved in vertebrate species although it does not belong to any known protein kinase groups. VLK is initially expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, but its expression is later confined to E-cadherin-negative mesenchyme. It is enriched in the Golgi apparatus and is thought to regulate the rate of protein export from the Golgi. Targeted disruption of VLK in mice leads to a defect in lung development and neonatal lethality. It has been suggested that mutations in VLK may be associated with the allergic condition atopy.

NCBI and Uniprot Product Information

• NCBI GI #: 238636897
• NCBI GeneID: 106522
• UniProt Accession #: Q5RJI4; Q3UZD3; Q8VBZ6; B6F136
• Molecular Weight: 48,988 Da
• NCBI Official Full Name: vertebrate lonesome kinase
• NCBI Official Synonym Full Names: protein kinase domain containing, cytoplasmic
• NCBI Official Symbol: Pkdcc
• NCBI Official Synonym Symbols: Vlk; MAd1; Adtk1; ESTM17; Sgk493; X83346; AI115348; AW548124
• NCBI Protein Information: extracellular tyrosine-protein kinase PKDCC; sugen kinase 493; vertebrate lonesome kinase; protein kinase-like protein SgK493; protein kinase domain-containing protein, cytoplasmic
• UniProt Protein Name: Extracellular tyrosine-protein kinase PKDCC
• Protein Family: Extracellular tyrosine-protein kinase
• UniProt Gene Name: Pkdcc
• UniProt Entry Name: PKDCC_MOUSE

Uniprot Description

PKDCC: Protein kinase which is required for longitudinal bone growth through regulation of chondrocyte differentiation. Involved in protein transport from the Golgi apparatus to the plasma membrane. Belongs to the protein kinase superfamily.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, Other; Other group; SgK493 family

Cellular Component: Golgi apparatus; extracellular region

Molecular Function: transferase activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: ossification; peptidyl-tyrosine phosphorylation; multicellular organismal development; multicellular organism growth; negative regulation of Golgi to plasma membrane protein transport; palate development; positive regulation of bone mineralization; limb morphogenesis; protein amino acid phosphorylation; bone mineralization; protein transport; positive regulation of chondrocyte differentiation; transport; cell differentiation; alveolus development; skeletal development; embryonic gut development; phosphorylation