Human Guanine nucleotide exchange factor VAV3 ELISA Kit | VAV3 elisa kit

Human Guanine nucleotide exchange factor VAV3 ELISA Kit

Cat. Num. AAA762491

• Reactivity: Human
• Synonyms: Guanine nucleotide exchange factor VAV3; Human Guanine nucleotide exchange factor VAV3 ELISA Kit; VAV3 (Guanine nucleotide exchange factor VAV3)/VAV-3; VAV3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of VAV3 . No significant cross-reactivity or interference between VAV3 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between VAV3 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 847

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VAV3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VAV3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VAV3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- VAV3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- VAV3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VAV3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VAV3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 120432044
• NCBI GeneID: 10451
• NCBI Accession #: NP_006104.4
• NCBI GenBank Nucleotide #: NM_006113.4
• Molecular Weight: 100,828 Da
• NCBI Official Full Name: guanine nucleotide exchange factor VAV3 isoform 1
• NCBI Official Synonym Full Names: vav guanine nucleotide exchange factor 3
• NCBI Official Symbol: VAV3
• NCBI Protein Information: guanine nucleotide exchange factor VAV3
• UniProt Protein Name: Guanine nucleotide exchange factor VAV3
• Protein Family: Guanine nucleotide exchange factor
• UniProt Gene Name: VAV3
• UniProt Synonym Gene Names: VAV-3
• UniProt Entry Name: VAV3_HUMAN

NCBI Description

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

VAV3: a guanine nucleotide exchange factor (GEF) that activates RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by activated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion. Interacts with the PH domain of APS. Interacts (via SH2 domains) with the phosphorylated form of EPHA2. Interacts with ROS1; constitutive interaction that mediates VAV3 phosphorylation. Down-regulated by EGF and TGF-beta. Four isoforms of the human protein are produced by alternative promoter. Isoform 1 and isoform 3 are widely expressed; both are expressed at very low levels in skeletal muscle. In keratinocytes, isoform 1 is less abundant than isoform 3. Isoform 3 is detected at very low levels, if any, in adrenal gland, bone marrow, spleen, fetal brain and spinal chord; in these tissues, isoform 1 is readily detectable.

Protein type: Nuclear receptor co-regulator; Adaptor/scaffold; Motility/polarity/chemotaxis; GEFs; GEFs, Rac/Rho; Actin-binding

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytosol; plasma membrane

Molecular Function: epidermal growth factor receptor binding; GTPase activator activity; guanyl-nucleotide exchange factor activity; metal ion binding; protein binding; Rac guanyl-nucleotide exchange factor activity; SH3/SH2 adaptor activity

Biological Process: angiogenesis; axon guidance; B cell receptor signaling pathway; blood coagulation; ephrin receptor signaling pathway; innate immune response; integrin-mediated signaling pathway; lamellipodium biogenesis; nerve growth factor receptor signaling pathway; neutrophil chemotaxis; platelet activation; positive regulation of apoptosis; positive regulation of B cell proliferation; positive regulation of cell adhesion; positive regulation of GTPase activity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of signal transduction; regulation of cell size; regulation of GTPase activity; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; response to DNA damage stimulus; response to drug; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway; vesicle fusion