Rabbit Estrogen Receptor alpha Polyclonal Antibody | anti-ESR1 antibody

Estrogen Receptor alpha (C-terminus) Antibody

Cat. Num. AAA474349

• Gene Names: ESR1; ER; ESR; Era; ESRA; NR3A1
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry
• Synonyms: Estrogen Receptor alpha; Polyclonal Antibody; Estrogen Receptor alpha (C-terminus) Antibody; Estrogen Receptor alpha (C-terminus); Estrogen Ra Cterm; anti-ESR1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: The antibody was affinity purified using ERalpha (C-terminus) peptide (without carrier). This antibody detects several forms of ERalpha ranging from 66 to 35kDa* on SDS-PAGE immunoblots of MCF-7 cells treated with pervanadate, and MDA-MB-231 cells.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-ESR1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:1000; ICC: 1:100; IP: 1:50; WB: 1:500
• Immunogen: ERalpha (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminus of human ERalpha. This sequence is well conserved in rat and mouse ERalpha, but is not found in ERbeta.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot image of human MCF-7 cells treated with pervanadate (1mM) for 30 min. (lanes 1-6). Some lanes of the blot were then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with mouse monoclonal anti-ERalpha (Tyr-537) phospho-specific (lanes 1 & 2), rabbit polyclonal anti-ERalpha (C-terminus) (lanes 3 & 4), and rabbit polyclonal anti-ERalpha (Tyr-537) phospho-specific (lanes 5 & 6).)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of Estrogen Receptor alpha in paraformaldehyde fixed and NP-40 permeabilized MDA-MB-231 cells. The cells were labeled with rabbit polyclonal anti-Estrogen Receptor alpha (EP5431). The antibody was detected using goat anti-rabbit DyLight 594.)

Related Product Information for anti-ESR1 antibody

Estrogen receptor alpha (ERalpha) is a member of the steroid receptor superfamily and its structure includes an N-terminal ligand-independent transactivation domain (AF-1), a highly conserved DNA binding domain, and a C-terminal ligand-dependent transactivation domain (AF-2). AF-1 and AF-2 activate transcription independently and synergistically, and act in a promoter- and cell-specific manner. Phosphorylation at multiple sites provides an important mechanism to regulate ERalpha activity. Ser-104, Ser-106, Ser-118, and Ser-167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERalpha activity. In addition to these sites, phosphorylation of Tyr-537 has been implicated in maximal hormone binding, dimerization, and transcriptional activity. Tyr-537, located in the AF-2 domain, is phosphorylated by c-Src leading to nuclear export of ERalpha and degradation. Thus, a variety of phosphorylation events control ERalpha activity.

References

Anbalagan M, Rowan BG (2015) Mol Cell Endocrin. 418(3):264.
Castoria, G. et al. (2012) Oncogene. 31:4868.

NCBI and Uniprot Product Information

• NCBI GI #: 62821794
• NCBI GeneID: 2099
• NCBI Accession #: NP_000116.2
• NCBI GenBank Nucleotide #: NM_000125.3
• UniProt Accession #: P03372; Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7
• Molecular Weight: 66,216 Da
• NCBI Official Full Name: estrogen receptor
• NCBI Official Synonym Full Names: estrogen receptor 1
• NCBI Official Symbol: ESR1
• NCBI Official Synonym Symbols: ER; ESR; Era; ESRA; NR3A1
• NCBI Protein Information: estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha delta 4 +49 isoform; nuclear receptor subfamily 3 group A member 1; estrogen
• UniProt Protein Name: Estrogen receptor
• Protein Family: Estrogen receptor
• UniProt Gene Name: ESR1
• UniProt Synonym Gene Names: ESR; NR3A1; ER
• UniProt Entry Name: ESR1_HUMAN

NCBI Description

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq, Jul 2008]

Uniprot Description

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus

Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity

Biological Process: estrogen receptor signaling pathway; positive regulation of nitric oxide biosynthetic process; uterus development; signal transduction; response to estradiol stimulus; regulation of apoptosis; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; antral ovarian follicle growth; positive regulation of nitric-oxide synthase activity; negative regulation of transcription factor activity; male gonad development; androgen metabolic process; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; gene expression; positive regulation of transcription factor activity; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling

Disease: Breast Cancer; Migraine With Or Without Aura, Susceptibility To, 1; Myocardial Infarction, Susceptibility To; Estrogen Resistance