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Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using DGKH Antibody. The picture on the right is blocked with the synthesized peptide.)

Rabbit anti-Human, Mouse DGKH Polyclonal Antibody | anti-DGKH antibody

DGKH Antibody

Gene Names
DGKH; DGKeta
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
DGKH; Polyclonal Antibody; DGKH Antibody; Diacylglycerol kinase eta; Diglyceride kinase eta; DGK-eta; DAG kinase eta; anti-DGKH antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
DGKH Antibody detects endogenous levels of total DGKH protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
1,084
Applicable Applications for anti-DGKH antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human DGKH.
Immunogen Range
771-820
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using DGKH Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using DGKH Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using DGKH Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using DGKH Antibody. The picture on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
134 kDa
NCBI Official Full Name
diacylglycerol kinase eta isoform 1
NCBI Official Synonym Full Names
diacylglycerol kinase eta
NCBI Official Symbol
DGKH
NCBI Official Synonym Symbols
DGKeta
NCBI Protein Information
diacylglycerol kinase eta
UniProt Protein Name
Diacylglycerol kinase eta
UniProt Gene Name
DGKH
UniProt Synonym Gene Names
DAG kinase eta; DGK-eta

NCBI Description

This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014]

Uniprot Description

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.

Research Articles on DGKH

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Product Notes

The DGKH dgkh (Catalog #AAA9505322) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DGKH Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DGKH can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the DGKH dgkh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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