Testing Data
Rabbit CSF-1R Polyclonal Antibody | anti-CSF-1R antibody
Rabbit anti CSF-1R/M-CSFR Polyclonal antibody
Gene Names
Csf1r; Fms; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; AI323359
Reactivity
Human, Rat, Mouse, Bovine
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Synonyms
CSF-1R; Polyclonal Antibody; Rabbit anti CSF-1R/M-CSFR Polyclonal antibody; Rabbit anti-CSF-1R; Colony stimulating factor 1R (CSF-1R); Macrophage colony stimulating factor receptor (M-CSFR); CD115; C-FMS.; anti-CSF-1R antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Bovine
Clonality
Polyclonal
Specificity
This antibody recognizes ~130 kDa of human CSF-1R protein. This antibody also reacts with mouse, rat. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-CSF-1R antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Antigen Preparation
A synthetic peptide corresponding to the C-terminus of human CSF-1R protein. This sequence is identical within human, mouse, rat, and bovine.
Positive Control
LNCaP
Cellular Location
Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-CSF-1R antibody
CD115, or Colony-stimulating factor-1 receptor (CSF-1R), is the major regulator of macrophage development and associated with epithelial cancers of the breast and ovary. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subquently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. The activated or phosphorylated form of CSF-1R has been associated with enhanced invasive and metastatic potential. The phosphorylation residues includeTyr 708, Tyr 723, Tyr 809 which correlate CSF-1R expression with changes in the growth and development of the cancer stage.
References
Eugene P. Toy et al. The Activated Macrophage Colony-Stimulating Factor (CSF-1) Receptor as a Predictor of Poor Outcome in Advanced Epithelial Ovarian Carcinoma. (2001). Gynecologic Oncology, 80 (2), 194-200.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~130 kDa
NCBI Official Full Name
Colony stimulating factor 1 receptor
NCBI Official Synonym Full Names
colony stimulating factor 1 receptor
NCBI Official Symbol
Csf1r
NCBI Official Synonym Symbols
Fms; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; AI323359
NCBI Protein Information
macrophage colony-stimulating factor 1 receptor; c-fms; CSF-1-R; OTTMUSP00000015851; proto-oncogene fms; proto-oncogene c-Fms
UniProt Protein Name
Macrophage colony-stimulating factor 1 receptor
UniProt Gene Name
Csf1r
UniProt Synonym Gene Names
Csfmr; Fms
UniProt Entry Name
CSF1R_MOUSE
Uniprot Description
Function: Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure: Interacts with INPPL1/SHIP2 and THOC5. Ref.8 Ref.9
Subcellular location: Membrane; Single-pass type I membrane protein.
Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 protein kinase domain.
Research Articles on CSF-1R
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Product Notes
The CSF-1R csf1r (Catalog #AAA462160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti CSF-1R/M-CSFR Polyclonal antibody reacts with Human, Rat, Mouse, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's CSF-1R can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 2-5 ug/ml. Researchers should empirically determine the suitability of the CSF-1R csf1r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CSF-1R, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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