Rabbit mTOR Polyclonal Antibody | anti-mTOR antibody

Rabbit anti mTOR Polyclonal antibody

Cat. Num. AAA462086

• Reactivity: Human, Rat, Mouse, Bovine, Canine, Chicken
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by Epitope Affinity Purification.
• Synonyms: mTOR; Polyclonal Antibody; Rabbit anti mTOR Polyclonal antibody; Rabbit anti-mTOR; Mammalian target of rapamycin (mTOR); RAPT1; FKBP12-Rapamycin; anti-mTOR antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Bovine, Canine, Chicken
• Clonality: Polyclonal
• Specificity: This antibody only recognizes ~290 kDa of human mTOR. It also reacts with mouse and rat mTOR protein. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-mTOR antibody: ELISA, Western Blot, Immunohistochemistry
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml
• Antigen Preparation: A synthetic peptide corresponding to the C-terminus of human mTOR protein. This sequence is identical among human, rat, mouse, chicken, dog and bovine species.
• Positive Control: MCF-7
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C -8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-mTOR antibody

The mammalian target of rapamycin (mTOR), also named FKBP12 rapamycin-associated protein (FRAP/RAFT/RAPT/SEP), is a Ser/Thr protein kinase that plays a crucial role in a nutrient-sensitive signalling pathway that regulates cell growth. TOR signalling is potently inhibited by rapamycin, through the direct binding of a FK506-binding protein 12 (FKBP12)/rapamycin complex to the TOR FRB domain, a segment amino terminal to the kinase catalytic domain. mTOR is involved in the regulation of cell growth through initiation of gene translation in response to nutrients such as amino acids (mainly leucine), growth factors, insulin and mitogens. mTOR initiates translation by activating the ribosomal p70S6k protein kinase (S6K1) and by inhibiting the eIF4E inhibitor 4E-BP1. mTOR is phosphorylated at serine 2448 via the phosphatidylinositol 3-kinase (PI3K)/Akt pathway and is autophosphorylated at serine 2481.

References

Noriko Oshiro, et al. Dissociation of raptor from mTOR is a mechanism of rapamycin-induced inhibition of mTOR function (2004), Genes to Cells (2004) 9, 359-366.