Rabbit FOXA3 Polyclonal Antibody | anti-FOXA3 antibody

Rabbit anti FOXA3/HNF3G Polyclonal antibody

Cat. Num. AAA462181

• Gene Names: FOXA3; FKHH3; HNF3G; TCF3G; MGC10179
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot
• Purity: The Rabbit IgG is purified by Epitope Affinity qPurification.
• Synonyms: FOXA3; Polyclonal Antibody; Rabbit anti FOXA3/HNF3G Polyclonal antibody; Rabbit anti-FOXA3; Forkhead Box A2 (FOXA3); Hepatocyte nuclear factor 3 gamma (HNF3G); anti-FOXA3 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody recognizes ~37 kDa of FOXA3 protein. It also reacts with human, mouse and rat. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Epitope Affinity qPurification.
• Form/Format: This affinity purified antibody is supplied in sterile phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-FOXA3 antibody: ELISA, Western Blot
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml
• Antigen Preparation: A synthetic peptide corresponding to the C-terminus of human FOXA3 protein. This sequence is identical to rat, mouse and human origins.
• Positive Control: Huh 7 cells
• Cellular Location: Nuclear
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-FOXA3 antibody

The forkhead box gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA1 plays an important role in breast cancer. The FOXA subfamily is composed of the transcription factors FOXA1, A2, A3. They were discovered as the regulators of hepatic genes. Foxa1 has been shown to be required for glucagon secretion in the pancreas, whereas Foxa2 is critical for the regulation of insulin secretion in pancreatic beta-cells. Foxa3 is important in the maintenance of glucose homeostasis, it is the dominating transcriptional regulator of GLUT2 expression in hepatocytes in vivo. In the normal activation of HNF-4alpha, HNF-1alpha, and PGC-1 induced by fasting is attenuated in mice lacking Foxa3.

References

Shen W et al. Foxa3 (hepatocyte nuclear factor 3gamma) is required for the regulation of hepatic GLUT2 expression and the maintenance of glucose homeostasis during a prolonged fast. 2001 Nov 16;276 (46):42812-7.

NCBI and Uniprot Product Information

• NCBI GI #: 160431602
• NCBI GeneID: 3171
• UniProt Accession #: P55318; Q53F16; Q9UMW9; A9LYI5
• Molecular Weight: ~37 kDa
• NCBI Official Full Name: forkhead box A3
• NCBI Official Synonym Full Names: forkhead box A3
• NCBI Official Symbol: FOXA3
• NCBI Official Synonym Symbols: FKHH3; HNF3G; TCF3G; MGC10179
• NCBI Protein Information: hepatocyte nuclear factor 3-gamma; HNF-3G; TCF-3G; HNF-3-gamma; forkhead box protein A3; transcription factor 3G; fork head-related protein FKH H3; hepatocyte nuclear factor 3, gamma
• UniProt Protein Name: Hepatocyte nuclear factor 3-gamma
• Protein Family: Hepatocyte nuclear factor
• UniProt Gene Name: FOXA3
• UniProt Synonym Gene Names: HNF3G; TCF3G
• UniProt Entry Name: FOXA3_HUMAN

NCBI Description

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. The crystal structure of a similar protein in rat has been resolved. [provided by RefSeq]

Uniprot Description

Function: Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites

By similarity. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; binds to and activates transcription from the G6PC promoter. Binds to the CYP3A4 promoter and activates its transcription in cooperation with CEBPA. Binds to the CYP3A7 promoter together with members of the CTF/NF-I family. Involved in regulation of neuronal-specific transcription. May be involved in regulation of spermatogenesis. Ref.9

Subunit structure: Interacts with FOXA2. Ref.10

Subcellular location: Nucleus Ref.11.

Tissue specificity: Expressed in erythroleukemia and hepatoma cell lines and in liver and pancreas. Not expressed in any other cell lines or tissues examined. Ref.1

Developmental stage: Detected in prenatal liver nuclear extracts (12.4-27 weeks estimated gestational age). Not detected in postnatal liver samples. Ref.11

Sequence similarities: Contains 1 fork-head DNA-binding domain.