Rabbit anti-Human VAMP7 Polyclonal Antibody | anti-VAMP7 antibody

Anti-VAMP7 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109455

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: VAMP7; Polyclonal Antibody; Anti-VAMP7 Antibody; Rabbit Polyclonal; VAMP7 Antibody; Rabbit PAb; Antigen Affinity Purified; vesicle-associated membrane protein 7; Anti-SYBL1 Antibody; Anti-TI-VAMP Antibody; Anti-TIVAMP Antibody; Anti-VAMP-7 Antibody; anti-VAMP7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human VAMP7
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-VAMP7 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human VAMP7 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human VAMP7 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-VAMP7 rabbit polyclonal antibody at 1:500 dilution Lane A: COLO205 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:24 kDa Observed band size:24 kDa (We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(VAMP7 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 4 uL anti-VAMP7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-VAMP7 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa Observed band size :24 kDa)

Related Product Information for anti-VAMP7 antibody

Tetanus neurotoxin-insensitive vesicular-associated membrane protein (TI-VAMP/VAMP7), a vesicular SNARE (Soluble N-ethylmaleimide-sensitive factor attachment protein receptors) expressed in several cell types including neurons, was shown to play a major role in exocytosis involved in neurite growth in cultured neurons. The expression of VAMP7 is found in testes, epididymis, seminal vesicles, prostatic tissues, penis, and urethra. VAMP7 protein mediates specific membrane fusions in intracellular trafficking and was reported to regulate autophagosome formation. VAMP7 regulates autophagy to maintain mitochondrial quality and insulin secretion in response to pathological stress in beta-cells. VAMP7 is involved in the fusion of late endocytic compartments with other membranes. One possible mechanism of VAMP7 delivery to these late compartments is via the AP3 trafficking adaptor.

References

Verraes A, et al. (2018) Comparative study of commercially available and homemade anti-vamp7 antibodies using crispr/cas9-depleted hela cells and vamp7 knockout mice. F1000Res 7 1649.Kent HM, et al. (2012) Structural basis of the intracellular sorting of the snare vamp7 by the ap3 adaptor complex. Dev Cell 22 (5): 979-988.Danglot L, et al. (2012) Absence of ti-vamp/vamp7 leads to increased anxiety in mice. J Neurosci 32 (6): 1962-1968.Aoyagi K, et al. (2016) Vamp7 regulates autophagy to maintain mitochondrial homeostasis and to control insulin secretion in pancreatic beta-cells. Diabetes 65 (6): 1648-1659.