Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RHBDF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Rabbit anti-Mouse, Rat RHBDF1 Polyclonal Antibody | anti-RHBDF1 antibody
RHBDF1 Rabbit pAb
Gene Names
RHBDF1; Dist1; hDist1; C16orf8; EGFR-RS; gene-89; gene-90
Reactivity
Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity purification
Synonyms
RHBDF1; Polyclonal Antibody; RHBDF1 Rabbit pAb; C16orf8; Dist1; EGFR-RS; gene-89; gene-90; hDist1; anti-RHBDF1 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
HRLPRRRKRESVAKMSFRAAAALMKGRSVRDGTFRRAQRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEVFESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMD
Applicable Applications for anti-RHBDF1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 200-400 of human RHBDF1 (NP_071895.3).
Positive Samples
Mouse lung, Mouse heart, Mouse kidney, Rat lung, Rat heart, Rat kidney
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RHBDF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RHBDF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Immunofluorescence (IF)
(Immunofluorescence analysis of L929 cells using RHBDF1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of L929 cells using RHBDF1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,401 Da
NCBI Official Full Name
inactive rhomboid protein 1
NCBI Official Synonym Full Names
rhomboid 5 homolog 1 (Drosophila)
NCBI Official Symbol
RHBDF1
NCBI Official Synonym Symbols
Dist1; hDist1; C16orf8; EGFR-RS; gene-89; gene-90
NCBI Protein Information
inactive rhomboid protein 1; iRhom1; p100hRho; rhomboid family 1; rhomboid family member 1; epidermal growth factor receptor-related protein; epidermal growth factor receptor, related sequence
UniProt Protein Name
Inactive rhomboid protein 1
UniProt Gene Name
RHBDF1
UniProt Synonym Gene Names
C16orf8; DIST1; IRHOM1; iRhom1
UniProt Entry Name
RHDF1_HUMAN
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Product Notes
The RHBDF1 rhbdf1 (Catalog #AAA9142993) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RHBDF1 Rabbit pAb reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RHBDF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the RHBDF1 rhbdf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HRLPRRRKRE SVAKMSFRAA AALMKGRSVR DGTFRRAQRR SFTPASFLEE DTTDFPDELD TSFFAREGIL HEELSTYPDE VFESPSEAAL KDWEKAPEQA DLTGGALDRS ELERSHLMLP LERGWRKQKE GAAAPQPKVR LRQEVVSTAG PRRGQRIAVP VRKLFAREKR PYGLGMVGRL TNRTYRKRID SFVKRQIEDM D. It is sometimes possible for the material contained within the vial of "RHBDF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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