Immunohistochemistry (IHC)
(Immunochemical staining of human VAMP4 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human VAMP4 Polyclonal Antibody | anti-VAMP4 antibody
Anti-VAMP4 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
VAMP4; Polyclonal Antibody; Anti-VAMP4 Antibody; Rabbit Polyclonal; VAMP4 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-RP4-560B9.3 Antibody; Anti-VAMP-4 Antibody; Anti-VAMP24 Antibody; anti-VAMP4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human VAMP4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-VAMP4 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human VAMP4 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human VAMP4 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human VAMP4 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human VAMP4 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human VAMP4 in cynomolgus cerebellum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human VAMP4 in cynomolgus cerebellum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-VAMP4 antibody
The predominant players in membrane fusion events are the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family of proteins. Vesicle-associated membrane protein 4 (VAMP4), one of SNARE family member, contains a dileucine motif and binds to the AP-1 (adaptor protein-1) subunit mu 1a, but not mu 1b, or the GGAs (Golgi-associated gamma ear containing ARF binding proteins). VAMP4 is enriched in the trans-Golgi network (TGN) and functions in traffic from the early and recycling endosomes to the TGN. The maintenance of Golgi ribbon structure requires normal retrograde trafficking from the early endosome to the TGN, which is likely to be mediated by the formation of VAMP4-containing SNARE complexes.
References
Steegmaier M, et al. (1999) Vesicle-associated membrane protein 4 is implicated in trans-golgi network vesicle trafficking. Mol Biol Cell 10 (6): 1957-1972.Shitara A, et al. (2013) Vamp4 is required to maintain the ribbon structure of the golgi apparatus. Mol Cell Biochem 380 (1-2): 11-21.Hinners I, et al. (2003) Ap-1 recruitment to vamp4 is modulated by phosphorylation-dependent binding of pacs-1. EMBO Rep 4 (12): 1182-1189.
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Product Notes
The VAMP4 (Catalog #AAA8110329) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-VAMP4 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VAMP4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the VAMP4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VAMP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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