Rabbit anti-Human Mst3b Polyclonal Antibody

Mst3b (Mammalian Ste20-like Kinase)

Cat. Num. AAA617998

• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Protein A and peptide affinity chromatography
• Synonyms: Mst3b; Polyclonal Antibody; Mst3b (Mammalian Ste20-like Kinase); Anti -Mst3b (Mammalian Ste20-like Kinase); anti-Mst3b antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects total level of endogenous Mst3b. Does not crossreact with Mst3, Mst1, Mst2 and Mst4
• Purity/Purification: Affinity Purified; Protein A and peptide affinity chromatography
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol

• Applicable Applications for anti-Mst3b antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide (KLH coupled) corresponding to the amino-terminal residues of human Mst3b
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

Western Blot (WB)

(Western Blot analysis of extracts from HeLa, NIH/3T3, C6 and Cos cells, using MBS617998)

Western Blot (WB)

(Western Blot analysis of extracts from HeLa cells treated with staurosporine for indicated times, using MBS617998)

Related Product Information for anti-Mst3b antibody

Mammalian Ste20-like kinases, including Mst1-4, belong to the germinal center kinase (GCK) family. The amino-terminal kinase domain of Mst is considerably homologous to the kinase domain of yeast Ste20 kinase and other p21-activated kinases (1). The carboxy-terminal region of Mst1 and Mst2 contains a dimerization and an inhibitory domain, while the carboxy-terminal region of Mst3 and Mst4 are not well characterized (1-3). Dimerization and phosphorylation at the activation loop regulates translocation of Mst1 from the cytosol to the nucleus (3). Growing evidence indicates that Mst1 and Mst2 are activated by apoptotic signals as well as other stress conditions (4-6). The full activation of Mst1 requires both phosphorylation and caspase-mediated cleavage (4). Activated Mst kinases may rely on p38 MAPK and JNK pathways to amplify apoptotic signals (5). Inhibitory phosphorylation is also reported. Phosphorylation at serine 327 of Mst1, which

Product Categories/Family for anti-Mst3b antibody

Antibodies; Abs to Protein Kinases