VRK3 recombinant protein

VRK3 recombinant protein

Cat. Num. AAA515272

• Applications: Western Blot
• Synonyms: VRK3; VRK3 recombinant protein

• Host: Sf9 insect cells
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 2129

• Applicable Applications for VRK3 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: Sf9 insect cells using baculovirus
• Source Note: Recombinant full-length human VRK3 was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for VRK3 recombinant protein

Recombinant full-length human VRK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: VRK3 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases which has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. Profound changes to the active site region underlie the loss of catalytic activity of VRK3 since it cannot bind ATP because of residue substitutions in the binding pocket (1). VRK3 is widely expressed in human tissues and the protein localizes to the nucleus. VRK3 regulates several transcription factors, nuclear envelope assembly, and chromatin condensation and is also required for cell cycle progression (2).

Product Categories/Family for VRK3 recombinant protein

Cellular Proteins; Signaling Proteins - Cellular Proteins

References

1. Scheeff, E D. et al: Structure of the pseudokinase VRK3 reveals a degraded catalytic site, a highly conserved kinase fold, and a putative regulatory binding site. Structure. 2009 Jan 14;17(1):128-38.
2. Sanz-Garci-a M, et.al: Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities. Mol Cell Proteomics. 2008 Nov;7(11):2199-214.

NCBI and Uniprot Product Information

• NCBI GI #: 71164885
• NCBI GeneID: 51231
• NCBI Accession #: NP_057524.3
• NCBI GenBank Nucleotide #: NM_016440
• UniProt Accession #: Q8IV63; Q502Y2; Q9P2V8; A6NEG5; A8KA53
• Molecular Weight: ~80 kDa
• NCBI Official Full Name: Homo sapiens vaccinia related kinase 3 (VRK3), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: vaccinia related kinase 3
• NCBI Official Symbol: VRK3
• NCBI Protein Information: inactive serine/threonine-protein kinase VRK3; vaccinia-related kinase 3; serine/threonine-protein pseudokinase VRK3
• UniProt Protein Name: Inactive serine/threonine-protein kinase VRK3
• Protein Family: Inactive serine/threonine-protein kinase
• UniProt Gene Name: VRK3
• UniProt Entry Name: VRK3_HUMAN

NCBI Description

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. This gene is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus

By similarity. Ref.6 Ref.12

Subunit structure: Interacts with DUSP3

By similarity. Interacts with RAN. Ref.7

Subcellular location: Nucleus Ref.6.

Sequence similarities: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.Contains 1 protein kinase domain.

Caution: Inactive as a kinase due to its inability to bind ATP (Ref.12).