Rabbit anti-Human USP7 Polyclonal Antibody | anti-USP7 antibody

USP7, ID (USP7, HAUSP, Ubiquitin Carboxyl-terminal Hydrolase 7, Deubiquitinating Enzyme 7, Herpes Virus-associated Ubiquitin-specific Protease, Ubiquitin Thioesterase 7, Ubiquitin-specific-processing Protease 7) (MaxLight 550)

Cat. Num. AAA6362022

• Gene Names: USP7; TEF1; HAUSP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: USP7; Polyclonal Antibody; ID (USP7; HAUSP; Ubiquitin Carboxyl-terminal Hydrolase 7; Deubiquitinating Enzyme 7; Herpes Virus-associated Ubiquitin-specific Protease; Ubiquitin Thioesterase 7; Ubiquitin-specific-processing Protease 7) (MaxLight 550); Ubiquitin carboxyl-terminal hydrolase 7; Deubiquitinating enzyme 7; Herpesvirus-associated ubiquitin-specific protease; Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7; anti-USP7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP7.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-USP7 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 320-348 in the central region of human USP7. Species sequence homology: rat
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in A2058 cell line lysates (35ug/lane) usingMBS6007955. This demonstrates that MBS6007955 detected the USP7 protein (arrow).)

Related Product Information for anti-USP7 antibody

Hydrolase that deubiquitinates target proteins such as FOXO4, TP53, MDM2, PTEN and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and proteasomal degradation. Deubiquitinates TP53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2, and also induces TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Contributes to the overall stabilization and trans-activation capability of the Herpes virus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection.

Product Categories/Family for anti-USP7 antibody

Antibodies; Ubiquitin

References

1. Sarkari, F., et al., J. Mol. Biol. 402(5): 825-837 (2010). 2. de Bie, P., et al., Biochem. Biophys. Res. Commun. 400(3): 389-395 (2010). 3. Maertens, G.N., et al., EMBO J. 29(15): 2553-2565 (2010). 4. Rose, J.E., et al., Mol. Med. 16(7-8): 247-253 (2010). 5. Tang, J., et al., Biochem. Biophys. Res. Commun. 393(3): 542-545 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 150378533
• NCBI GeneID: 7874
• NCBI Accession #: NP_003461.2
• NCBI GenBank Nucleotide #: NM_003470.2
• UniProt Accession #: Q93009; A6NMY8; B7Z815; H0Y3G8
• Molecular Weight: – Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 7 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 7 (herpes virus-associated)
• NCBI Official Symbol: USP7
• NCBI Official Synonym Symbols: TEF1; HAUSP
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 7; Herpes virus-associated ubiquitin-specific protease; deubiquitinating enzyme 7; herpesvirus-associated ubiquitin-specific protease; ubiquitin specific protease 7 (herpes virus-associated); ubiquitin thioesterase 7
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 7
• UniProt Gene Name: USP7
• UniProt Synonym Gene Names: HAUSP
• UniProt Entry Name: UBP7_HUMAN

Uniprot Description

USP7: an enzyme that removes ubiquitin from its protein substrates. Binds to the herpes virus protein VMW110 that may therefore modulate its substrate specificity or activity to stabilize viral proteins. Regulates MDM2 and has a dynamic role in the p53-MDM2 pathway. Is reported to deubiquitinate and stabilize p53.

Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nuclear body; PML body; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein binding; p53 binding; cysteine-type endopeptidase activity; ubiquitin protein ligase binding; ubiquitin-specific protease activity; transcription factor binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; maintenance of DNA methylation; viral reproduction; regulation of transcription factor activity; inhibition of NF-kappaB transcription factor; transcription-coupled nucleotide-excision repair; multicellular organismal development; histone deubiquitination