Western Blot (WB)
(USP7 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the USP7 antibody detected the USP7 protein (arrow).)
Rabbit USP7 Polyclonal Antibody | anti-USP7 antibody
USP7 Antibody (Center)
Gene Names
USP7; TEF1; HAUSP
Reactivity
Human (Predicted Reactivity: Rat)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
USP7; Polyclonal Antibody; USP7 Antibody (Center); Ubiquitin carboxyl-terminal hydrolase 7; Deubiquitinating enzyme 7; Herpesvirus-associated ubiquitin-specific protease; Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7; HAUSP; anti-USP7 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This USP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-348 amino acids from the Central region of human USP7.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
320-348
Sequence Length
–
Applicable Applications for anti-USP7 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(USP7 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the USP7 antibody detected the USP7 protein (arrow).)
Western Blot (WB)
(USP7 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the USP7 antibody detected the USP7 protein (arrow).)
Related Product Information for anti-USP7 antibody
Hydrolase that deubiquitinates target proteins such as FOXO4, TP53, MDM2, PTEN and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and proteasomal degradation. Deubiquitinates TP53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2, and also induces TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection.
Product Categories/Family for anti-USP7 antibody
References
Sarkari, F., et al. J. Mol. Biol. 402(5):825-837(2010)
de Bie, P., et al. Biochem. Biophys. Res. Commun. 400(3):389-395(2010)
Maertens, G.N., et al. EMBO J. 29(15):2553-2565(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Tang, J., et al. Biochem. Biophys. Res. Commun. 393(3):542-545(2010)
de Bie, P., et al. Biochem. Biophys. Res. Commun. 400(3):389-395(2010)
Maertens, G.N., et al. EMBO J. 29(15):2553-2565(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Tang, J., et al. Biochem. Biophys. Res. Commun. 393(3):542-545(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
128302
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 7 isoform 1
NCBI Official Synonym Full Names
ubiquitin specific peptidase 7 (herpes virus-associated)
NCBI Official Symbol
USP7
NCBI Official Synonym Symbols
TEF1; HAUSP
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 7
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 7
UniProt Gene Name
USP7
UniProt Synonym Gene Names
HAUSP
UniProt Entry Name
UBP7_HUMAN
Uniprot Description
USP7: an enzyme that removes ubiquitin from its protein substrates. Binds to the herpes virus protein VMW110 that may therefore modulate its substrate specificity or activity to stabilize viral proteins. Regulates MDM2 and has a dynamic role in the p53-MDM2 pathway. Is reported to deubiquitinate and stabilize p53.
Protein type: Ubiquitin-specific protease; EC 3.4.19.12; Protease; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: nuclear body; PML body; cytosol; nucleus
Molecular Function: protein C-terminus binding; protein binding; p53 binding; ubiquitin protein ligase binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; transcription factor binding
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of transcription factor activity; viral reproduction; maintenance of DNA methylation; inhibition of NF-kappaB transcription factor; multicellular organismal development; transcription-coupled nucleotide-excision repair; histone deubiquitination
Research Articles on USP7
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Product Notes
The USP7 usp7 (Catalog #AAA9208767) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 320-348. The USP7 Antibody (Center) reacts with Human (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's USP7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the USP7 usp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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