Mouse interleukin 4 induced 1 ELISA Kit | IL4I1 elisa kit

Mouse L-amino-acid oxidase, IL4I1 ELISA Kit

Cat. Num. AAA941525

• Reactivity: Mouse
• Synonyms: interleukin 4 induced 1; Mouse L-amino-acid oxidase; IL4I1 ELISA Kit; Mouse L-amino-acid oxidase (IL4I1) ELISA kit; FIG1; Fig-1 protein; interleukin 4 induced protein 1; interleukin four induced 1; IL4I1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse IL4I1. No significant cross-reactivity or interference between mouse IL4I1 and analogues was observed.
• Sequence Length: 638

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: <3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL4I1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL4I1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL4I1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL4I1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL4I1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 283484016
• NCBI GeneID: 100328588
• NCBI Accession #: NP_001164495.1
• NCBI GenBank Nucleotide #: NM_001171024.1
• UniProt Accession #: O09046; Q1LZI6; Q3T9N9; Q3U7S6; Q3V0K2; Q6Y632; Q6YBV6; Q6YDI8; Q8R2G8; Q9CXK7
• Molecular Weight: 70,191 Da
• NCBI Official Full Name: Nup62-Il4i1 protein
• NCBI Official Symbol: Nup62-il4i1
• NCBI Protein Information: Nup62-Il4i1 protein
• UniProt Protein Name: L-amino-acid oxidase
• UniProt Gene Name: Il4i1
• UniProt Synonym Gene Names: Fig1; LAAO; LAO; IL4-induced protein 1; mFIG1
• UniProt Entry Name: OXLA_MOUSE

NCBI Description

This locus represents naturally-occurring readthrough transcription between Nup62 (nucleoporin 62) and Il4i1 (interleukin 4 induced 1). It encodes a protein sharing similarity with the protein encoded by Il4i1, but with a longer and distinct N-terminus. [provided by RefSeq, Aug 2015]

Uniprot Description

IL4I1: Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation. Belongs to the flavin monoamine oxidase family. FIG1 subfamily. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - phenylalanine; Oxidoreductase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - cysteine and methionine; Secreted; Secreted, signal peptide; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 1.4.3.2

Cellular Component: lysosome

Molecular Function: L-amino-acid oxidase activity

Biological Process: aromatic amino acid family metabolic process