Phospholipase A1 (PLA1) Recombinant Protein | PLA1 recombinant protein

Recombinant Phospholipase A1 (PLA1)

Cat. Num. AAA2010246

• Gene Names: PLA1A; PSPLA1; PS-PLA1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Phospholipase A1 (PLA1); Recombinant Phospholipase A1 (PLA1); PLA1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- LV EQGGVKIEPL PKEVKVYLLT TSSAPYCMHH SLVEFHLKEL RNKDTNIEVT FLSSNITSSS KITIPKQQRY GKGIIAHATP QCQINQVKFK FQSSNRVWKK DRTTIIGKFC TALLPVNDRE KMVCLPEPVN LQASVTVSCD LK
• Sequence Length: 440

• Applicable Applications for PLA1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Leu309~Lys452 (Accession # Q53H76) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 332688256
• NCBI GeneID: 51365
• NCBI Accession #: NP_001193889.1
• NCBI GenBank Nucleotide #: NM_001206960.1
• UniProt Accession #: Q53H76; O95991; Q86WX6; Q9UPD2; B2R8V2; B4DXA2
• Molecular Weight: 17.8kDa
• NCBI Official Full Name: phospholipase A1 member A isoform 2
• NCBI Official Synonym Full Names: phospholipase A1 member A
• NCBI Official Symbol: PLA1A
• NCBI Official Synonym Symbols: PSPLA1; PS-PLA1
• NCBI Protein Information: phospholipase A1 member A; phosphatidylserine-specific phospholipase A1alpha
• UniProt Protein Name: Phospholipase A1 member A
• Protein Family: Phospholipase
• UniProt Gene Name: PLA1A
• UniProt Synonym Gene Names: NMD; PSPLA1; PS-PLA1
• UniProt Entry Name: PLA1A_HUMAN

NCBI Description

The protein encoded by this gene is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine in liposomes. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]

Uniprot Description

PLA1A: Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1- acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production. Belongs to the AB hydrolase superfamily. Lipase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.-; Secreted, signal peptide; Phospholipase; Secreted

Chromosomal Location of Human Ortholog: 3q13.13-q13.2

Cellular Component: acrosomal membrane

Molecular Function: phospholipase A1 activity

Biological Process: phosphatidylserine metabolic process; lipid metabolic process; lipid catabolic process