Testing Data
Rabbit Rub1 Polyclonal Antibody | anti-Rub1 antibody
Anti-Rub1 (Yeast) Antibody
Applications
Western Blot, ELISA
Purity
Affinity Purified
Synonyms
Rub1; Polyclonal Antibody; Anti-Rub1 (Yeast) Antibody; anti-Rub1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Purity/Purification
Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
77
Applicable Applications for anti-Rub1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
This purified polyclonal antibody reacts with yeast Rub1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-Rub1 antibody
Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBL's) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBL's are SUMO, Rub1 (also called Nedd8), Apg12, and Hub1. Proteins of the second class include parkin, RAD23, and DSK2, and are designated ubiquitin-domain proteins (UDP's). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBL's, UDP's are not proteolytically processed or conjugated to other proteins. Rub1, and the corresponding human homolog Nedd8, are activated by the E1 ubiquitin-activating enzyme UBA2, which forms isopeptide linkages between thioesters. Nedd8 shows 80% homology to ubiquitin. The best known targets of Rub1 modification are members of the cullin family. Cullins are subunits of an E3-ubiquitin ligase complex called the Skp1/Cul1/Cdc53-F-box (SCF). The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein. Rub1 modification may regulate SCF function or localization.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Rub1 (Catalog #AAA4156653) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Rub1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). This purified polyclonal antibody reacts with yeast Rub1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. Researchers should empirically determine the suitability of the Rub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rub1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
