Testing Data
Goat anti-Human Fetuin Polyclonal Antibody | anti-AHSG antibody
Anti-Human Fetuin (2-HS Glycoprotein) Antibody
Gene Names
AHSG; FETUA; FETUIN
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity Purified
Synonyms
Fetuin; Polyclonal Antibody; Anti-Human Fetuin (2-HS Glycoprotein) Antibody; Human Fetuin; anti-AHSG antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.
Purity/Purification
Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
362
Applicable Applications for anti-AHSG antibody
Western Blot (WB), ELISA (EIA)
Application Notes
This purified polyclonal antibody reacts with human fetuin by ELISA and western blot. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. A doublet band or slightly lower molecular weight band (see Figure 1, lane 1) may be visible by western blot due to proteolytic processing, variable glycosylation and/or phosphorylation. Proteolytic processing may include the removal of a 40 amino acid residue bridging peptide from the A and B chains of fetuin in vivo.
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with a recombinant human fetuin (a2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for anti-AHSG antibody
Human fetuin (2-Heremans-Schmid-glycoprotein or a2-HS-glycoprotein) is a major plasma glycoprotein predominantly synthesized in the liver. Human fetuin is named after its bovine homolog. Fetuins are found in most mammals. Human fetuin is a negative acute-phase protein; normal circulating levels in adults (300-600 ug/ml) fall significantly (30-50%) during injury and infection. The biological role of fetuin is unknown, although it has been implicated as an immunomodulator that can participate in stimulation of bacterial phagocytosis by neutrophils and promotion of endocytosis by mouse macrophages. Hepatocytes are the principal cell source of circulating fetuin, but it also is expressed by monocyte/macrophages. Fetuins occur in large amounts in blood and cerebrospinal fluid and accumulate to high concentrations in calcified bone. The fetuin promoter region has several potential interleukin 6-responsive elements, and its synthesis is down-regulated during injury and inflammation. Fetuin is an acidic glycoprotein with three Nlinked and three O-linked oligosaccharide chains, whose terminal sugar residues are rich in sialic acid (Nacetylneuraminic acid), contributing to its net negative charge. A role for fetuin as a carrier of bioactive molecules has been proposed based on observations that it binds and carries Ca2+ ion. Fetuin is implicated in bone remodeling, immune function and may play a role in tumor progression of certain cell types. Fetuin plays a role as an anti-inflammatory agent by suppressing the release of TNF from stimulated macrophages. Fetuin also interacts with members of the matrix metalloprotease family of zinc dependent secreted transmembrane proteins that degrade basement membranes and extracellular matrix components. The biological activity of fetuin is mediated through its direct interaction with other proteins. Fetuin down-regulates a number of receptor tyrosine kinase family members. A motif within fetuin has homology to the TGF-b receptor type II. Circulating human plasma fetuin is partly phosphorylated which implies that phosphorylated fetuin may have a physiological function in vivo. Human fetuin isolated from plasma is a two-chain molecule consisting of a A-chain of 322 amino acid residues (53 kDa) and a B-chain of 27 residues (5 kDa). The carboxy terminal 40 amino acids of the A chain constitute a bridging peptide that may be removed proteolytically in vivo. A single mRNA encodes both the A and B chains. The apparent molecular weight of intact human fetuin is 58 kDa.
NCBI and Uniprot Product Information
Research Articles on AHSG
Similar Products
Product Notes
The AHSG (Catalog #AAA4156800) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human Fetuin (2-HS Glycoprotein) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Fetuin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). This purified polyclonal antibody reacts with human fetuin by ELISA and western blot. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. A doublet band or slightly lower molecular weight band (see Figure 1, lane 1) may be visible by western blot due to proteolytic processing, variable glycosylation and/or phosphorylation. Proteolytic processing may include the removal of a 40 amino acid residue bridging peptide from the An and B chains of fetuin in vivo. Researchers should empirically determine the suitability of the AHSG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fetuin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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