Rabbit UCHL1, UCHL3 Polyclonal Antibody | anti-Uchl3 antibody

UCHL1, UCHL3 (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, UCH-L1, Neuron Cytoplasmic Protein 9.5, PGP 9.5, PGP9.5, Ubiquitin Thioesterase L1/Ubiquitin Carboxyl-terminal Hydrolase Isozyme L3, UCH-L3, Ubiquitin Thioesterase L3)

Cat. Num. AAA648203

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: UCHL1; UCHL3; Polyclonal Antibody; UCHL3 (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1; UCH-L1; Neuron Cytoplasmic Protein 9.5; PGP 9.5; PGP9.5; Ubiquitin Thioesterase L1/Ubiquitin Carboxyl-terminal Hydrolase Isozyme L3; UCH-L3; Ubiquitin Thioesterase L3); Anti -UCHL1; anti-Uchl3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UCHL1 and UCHL3. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in HEPES, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.

• Applicable Applications for anti-Uchl3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:2000
• Immunogen: Recombinant corresponding to human UCHL1, expressed in E.coli.
• Positive Control: SK-N-MC cells
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunoblot (IB)

(Immunoblot analysis of cell lysates Lane 1: SK-N-MC cell lysate Lane 2: IMR-32 cell lysate Lane 3: Mouse brain Lane 4: Rat brain)

Related Product Information for anti-Uchl3 antibody

The two ubiquitin C-terminal hydrolase (UCH) enzymes, UCHL1 and UCHL3, deubiquitinate ubiquitin-protein conjugates and control the cellular balance of ubiquitin. UCHL1 and UCHL3 are both small proteins of ~220aa that share more than 40% aa sequence identity. UCHL3 is universally expressed in all tissues, while UCHL1 is expressed exclusively in neuronal tissue, testis and ovary. The activity of UCHL3 is more than 200 fold higher than UCHL1 when a fluorogenic ubiquitin substrate is used. UCHL1 associates with monoubiquitin and UCHL3 binds to Nedd8, ubiquitin-like protein. UCHL1 and UCHL3 play a role in the regulation of neuronal development and spermatogenesis. UCHL1 is involved in the pathogenesis of Parkinson's disease (PD) and Alzheimer's disease (AD). Down-regulation and extensive oxidative modification of UCHL1 have been observed in the brains of AD patients as well as PD patients. Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Product Categories/Family for anti-Uchl3 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 139948802
• NCBI GeneID: 50933
• NCBI Accession #: NP_057932.2
• NCBI GenBank Nucleotide #: NM_016723.2
• UniProt Accession #: Q9JKB1; Q9EQX7
• Molecular Weight: 26,152 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L3
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• NCBI Official Symbol: Uchl3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L3; UCH-L3; ubiquitin thiolesterase; ubiquitin thioesterase L3
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: Uchl3
• UniProt Synonym Gene Names: UCH-L3
• UniProt Entry Name: UCHL3_MOUSE

Uniprot Description

Function: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome. Ref.5 Ref.6 Ref.7 Ref.8 Ref.10 Ref.12

Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulation: Inhibited by monoubiquitin and diubiquitin

By similarity.

Subunit structure: Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates

By similarity.

Subcellular location: Cytoplasm Ref.2 Ref.8.

Tissue specificity: Ubiquitously expressed, with highest levels in brain, liver, heart, thymus, kidney and testis. Highly expressed in the cauda epididymidis, in meiotic pachytene spermatocytes and post-meiotic spematids. In the retina, enriched in the photoreceptor inner segment. Ref.1 Ref.2 Ref.6 Ref.7

Developmental stage: Expressed at E8.5 in structures required for skeletal patterning. Highly expressed at E11, and decreases markedly from E15. Ref.1 Ref.4

Disruption phenotype: Mice have no developmental defects, are fertile, and show normal T-cell differentiation. They have normal anxiety, locomotor behavior, motor function and synaptic transmission, but show defects in spatial learning and working memory. Exhibit stress-related effects with profound apoptosis-mediated germ cell loss and also, prominent retinal degeneration with photoreceptor cell apoptosis and mitochondrial oxidative stress. Mice show reduced capacity for adipocyte differentiation and impaired insulin responses. Ref.5 Ref.6 Ref.7 Ref.10 Ref.11

Sequence similarities: Belongs to the peptidase C12 family.