Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using UCHL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Rabbit anti-Human, Mouse UCHL1 Polyclonal Antibody | anti-UCHL1 antibody
UCHL1 Rabbit pab Antibody
Gene Names
UCHL1; PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Affinity purification
Synonyms
UCHL1; Polyclonal Antibody; UCHL1 Rabbit pab Antibody; PARK5; PGP9.5; Uch-L1; anti-UCHL1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
223
Applicable Applications for anti-UCHL1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot (WB): 1/1000-2000
Immunohistochemistry (IHC): 1/50
Immunocytochemistry (ICC): 1/50
Immunoprecipitation (IP): 1/50
Immunohistochemistry (IHC): 1/50
Immunocytochemistry (ICC): 1/50
Immunoprecipitation (IP): 1/50
Immunogen
A synthetic -peptide of human UCHL1
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol,
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using UCHL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using UCHL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using UCHL1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using UCHL1 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-UCHL1 antibody
Rabbit polyclonal to Human UCHL1
Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs) (1,2). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM. UCHL1, UCHL3, UCHL5/UCH37, and BRCA-1-associated protein-1 (BAP1) belong to the UCH family of DUBs, which all posses a conserved catalytic domain (UCH domain) of about 230 amino acids. UCHL5 and BAP1 have unique extended C-terminal tails. UCHL1 is abundantly expressed in neuronal tissues and testes, while UCHL3 expression is more widely distributed (3,4). Although UCHL1 and UCHL3 are the most closely related UCH family members with about 53% identity, their biochemical properties differ in that UCHL1 binds monoubiquitin and UCHL3 shows dual specificity toward both ubiquitin (Ub) and NEDD8, a Ub-like molecule. In particular, UCHL3 functions as a Ub hydrolase involved in the processing of both Ub precursors and ubiquitinated substrates, generating free monomeric Ub. This is accomplished through the ability of UCHL3 to recognize and hydrolyze isopeptide bonds at the C-terminal glycine of either Ub or NEDD8 (5-7). Recent functional studies have identified UCH-L3 as a critical regulator of adipogenesis through its ability to promote IGF-IR and insulin receptor signaling (8). Furthermore, UCHL3 has been shown to promote deubiquitination, recycling, and cell surface expression of the epithelial sodium channel (9).
Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs) (1,2). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM. UCHL1, UCHL3, UCHL5/UCH37, and BRCA-1-associated protein-1 (BAP1) belong to the UCH family of DUBs, which all posses a conserved catalytic domain (UCH domain) of about 230 amino acids. UCHL5 and BAP1 have unique extended C-terminal tails. UCHL1 is abundantly expressed in neuronal tissues and testes, while UCHL3 expression is more widely distributed (3,4). Although UCHL1 and UCHL3 are the most closely related UCH family members with about 53% identity, their biochemical properties differ in that UCHL1 binds monoubiquitin and UCHL3 shows dual specificity toward both ubiquitin (Ub) and NEDD8, a Ub-like molecule. In particular, UCHL3 functions as a Ub hydrolase involved in the processing of both Ub precursors and ubiquitinated substrates, generating free monomeric Ub. This is accomplished through the ability of UCHL3 to recognize and hydrolyze isopeptide bonds at the C-terminal glycine of either Ub or NEDD8 (5-7). Recent functional studies have identified UCH-L3 as a critical regulator of adipogenesis through its ability to promote IGF-IR and insulin receptor signaling (8). Furthermore, UCHL3 has been shown to promote deubiquitination, recycling, and cell surface expression of the epithelial sodium channel (9).
References
1. Nijman, S.M. et al. (2005) Cell 123, 773-86. 2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613. 3. Leroy, E. et al. (1998) Nature 395, 451-2. 4. Kurihara, L.J. et al. (2001) Hum Mol Genet 10, 1963-70. 5. Osaka, H. et al. (2003) Hum Mol Genet 12, 1945-58. 6. Wada, H. et al. (1998) Biochem Biophys Res Commun 251, 688-92. 7. Kwon, J. (2007) Exp Anim 56, 71-7. 8. Suzuki, M. et al. (2009) Endocrinology 150, 5230-9. 9. Butterworth, M.B. et al. (2007) J Biol Chem 282, 37885-93.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
223
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Official Synonym Full Names
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
NCBI Official Symbol
UCHL1
NCBI Official Synonym Symbols
PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Protein Family
UniProt Gene Name
UCHL1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
UniProt Entry Name
UCHL1_HUMAN
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Product Notes
The UCHL1 uchl1 (Catalog #AAA126017) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UCHL1 Rabbit pab Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UCHL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot (WB): 1/1000-2000 Immunohistochemistry (IHC): 1/50 Immunocytochemistry (ICC): 1/50 Immunoprecipitation (IP): 1/50. Researchers should empirically determine the suitability of the UCHL1 uchl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UCHL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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