Western Blot (WB)
(Western blot analysis of anti-GLK Pab in Hela cell lysate. Dilution for anti-GLK was 1:100; dilution for secondary goat anti-rabbit-HRP was 1:7000. A chemiluminescent kit was used for development of the Western blot. Data and protocol courtesy of Dr. Richard Lu, Partners HealthCare System at Harvard University.)
Rabbit anti-Human GLK Polyclonal Antibody | anti-glk antibody
GLK, CT (Germinal Center Kinase Related Protein Kinase, Mitogen-activated Protein Kinase Kinase Kinase Kinase 3, MAP4K3, MAPKKKK3, MAPK/ERK Kinase Kinase Kinase 3, MEK Kinase Kinase 3, MEKKK 3, RAB8IPL1)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified by ammonium sulfate precipitation.
Synonyms
GLK; Polyclonal Antibody; CT (Germinal Center Kinase Related Protein Kinase; Mitogen-activated Protein Kinase Kinase Kinase Kinase 3; MAP4K3; MAPKKKK3; MAPK/ERK Kinase Kinase Kinase 3; MEK Kinase Kinase 3; MEKKK 3; RAB8IPL1); Anti -GLK; anti-glk antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GLK.
Purity/Purification
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Concentration
0.68mg/ml (varies by lot)
Applicable Applications for anti-glk antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended Dilution: WB: 1:1000, IHC (FFPE): 1:50-1:100 - Other applications not tested. Optimal dilutions to be determined by the researcher.
Grade
Purified
Immunogen
KLH-conugated synthetic peptide mapping to a fragment of residues within amino acids 423-453 in the C-terminal region of human GLK.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-GLK Pab in Hela cell lysate. Dilution for anti-GLK was 1:100; dilution for secondary goat anti-rabbit-HRP was 1:7000. A chemiluminescent kit was used for development of the Western blot. Data and protocol courtesy of Dr. Richard Lu, Partners HealthCare System at Harvard University.)
Western Blot (WB)
(Western blot analysis of anti-GLK Pab in Hela cell lysate. Dilution for anti-GLK was 1:100; dilution for secondary goat anti-rabbit-HRP was 1:7000. A chemiluminescent kit was used for development of the Western blot. Data and protocol courtesy of Dr. Richard Lu, Partners HealthCare System at Harvard University.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-glk antibody
GLK is a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase kinase 1 (MEKK1). This gene previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3).
Product Categories/Family for anti-glk antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
Glk
NCBI Official Symbol
glk
NCBI Protein Information
Glk
Protein Family
Similar Products
Product Notes
The glk (Catalog #AAA623463) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GLK, CT (Germinal Center Kinase Related Protein Kinase, Mitogen-activated Protein Kinase Kinase Kinase Kinase 3, MAP4K3, MAPKKKK3, MAPK/ERK Kinase Kinase Kinase 3, MEK Kinase Kinase 3, MEKKK 3, RAB8IPL1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GLK can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended Dilution: WB: 1:1000, IHC (FFPE): 1:50-1:100 - Other applications not tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the glk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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