Western Blot (WB)
(Western blot analysis of extracts from various cell types using MBS618623 UCHL3.)
Rabbit Ubiquitin C-Terminal Hydrolase L3 Polyclonal Antibody | anti-Uchl3 antibody
Ubiquitin C-Terminal Hydrolase L3 (UCH-L3, UCH-30kD)
Purified by peptide affinity chromatography.
Purified by peptide affinity chromatography.
Dilution: Western Blot: 1:1000
Western Blot (WB)
(Western blot analysis of extracts from various cell types using MBS618623 UCHL3.)
Western Blot (WB)
(Western blot analysis of extracts from various cell types using MBS618623 UCHL3.)
NCBI and Uniprot Product Information
Uniprot Description
UCHL3: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates. Highly expressed in heart, skeletal muscle, and testis. Inhibited by monoubiquitin and diubiquitin. Belongs to the peptidase C12 family.
Protein type: Protease; EC 3.4.19.12; Ubiquitin conjugating system
Cellular Component: nucleoplasm; cytoplasm; intracellular; nucleus
Molecular Function: peptidase activity; ubiquitin binding; hydrolase activity; ubiquitin-specific protease activity; cysteine-type peptidase activity
Biological Process: ubiquitin-dependent protein catabolic process; protein deubiquitination; cellular response to insulin stimulus; eating behavior; retina development in camera-type eye; positive regulation of fat cell differentiation; protein catabolic process; proteolysis; adult walking behavior
Research Articles on Uchl3
Similar Products
Product Notes
The Uchl3 uchl3 (Catalog #AAA618623) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ubiquitin C-Terminal Hydrolase L3 (UCH-L3, UCH-30kD) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin C-Terminal Hydrolase L3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the Uchl3 uchl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin C-Terminal Hydrolase L3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
