IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of CD103/Itgae using anti-CD103/Itgae antibody (AAA126483).CD103/Itgae was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CD103/Itgae Antibody (AAA126483) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
Rabbit anti-Mouse, Rat CD103/Itgae Polyclonal Antibody | anti-ITGAE antibody
Anti-CD103/Itgae Antibody Picoband
Gene Names
Itgae; CD103; aM290; alpha-E1; A530055J10; alpha-M290
Reactivity
Mouse, Rat
Applications
ELISA, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
CD103/Itgae, Antibody; Anti-CD103/Itgae Antibody Picoband; anti-ITGAE antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-ITGAE antibody
ELISA, IHC (Immunohistochemistry)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived mouse CD103/Itgae recombinant protein (Position: F20-E1061).
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of CD103/Itgae using anti-CD103/Itgae antibody (AAA126483).CD103/Itgae was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CD103/Itgae Antibody (AAA126483) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of CD103/Itgae using anti-CD103/Itgae antibody (AAA126483).CD103/Itgae was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CD103/Itgae Antibody (AAA126483) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 1. IHC analysis of CD103/Itgae using anti-CD103/Itgae antibody (AAA126483).CD103/Itgae was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CD103/Itgae Antibody (AAA126483) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 1. IHC analysis of CD103/Itgae using anti-CD103/Itgae antibody (AAA126483).CD103/Itgae was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CD103/Itgae Antibody (AAA126483) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
Related Product Information for anti-ITGAE antibody
ITGAE(INTEGRIN, ALPHA-E), also known as CD103, is an integrin protein that in human is encoded by the ITGAE gene. The ITGAE gene is mapped to chromosome 17p13 by inclusion within a BAC contig. ITGAE is expressed widely on intraepithelial lymphocyte(IEL) T cells(both alphabeta T cells and gammadelta T cells) and on some peripheral regulatory T cells(Tregs) which is important for decreasing the immune response and appears to play a crucial role in the prevention of autoimmune diseases. ITGAE shares overall homology with other alpha integrins but has a unique 55-amino acid "extra" region located just N-terminal to the I domain that contains 18 consecutive charged residues and a proteolytic cleavage site.
Product Categories/Family for anti-ITGAE antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
128,906 Da
NCBI Official Full Name
integrin alpha-E isoform 1
NCBI Official Synonym Full Names
integrin alpha E, epithelial-associated
NCBI Official Symbol
Itgae
NCBI Official Synonym Symbols
CD103; aM290; alpha-E1; A530055J10; alpha-M290
NCBI Protein Information
integrin alpha-E; integrin alpha M290
UniProt Protein Name
Integrin alpha-E
UniProt Gene Name
Itgae
UniProt Entry Name
ITAE_MOUSE
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Product Notes
The ITGAE itgae (Catalog #AAA126483) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD103/Itgae Antibody Picoband reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD103/Itgae can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ITGAE itgae for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD103/Itgae, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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