Rabbit anti-Human OTUD6B Polyclonal Antibody | anti-OTUD6B antibody

Anti-OTUD6B Antibody, Rabbit Polyclonal

Cat. Num. AAA8109449

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: OTUD6B; Polyclonal Antibody; Anti-OTUD6B Antibody; Rabbit Polyclonal; OTUD6B Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-CGI-77 Antibody; Anti-DUBA-5 Antibody; Anti-DUBA5 Antibody; Anti-IDDFSDA Antibody; anti-OTUD6B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human OTUD6B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-OTUD6B antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human OTUD6B fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human OTUD6B fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-OTUD6B rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:36 kDa)

Immunoprecipitation (IP)

(OTUD6B was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 4 uL anti-OTUD6B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-OTUD6B rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa Observed band size :36 kDa)

Related Product Information for anti-OTUD6B antibody

Deubiquitinating enzymes (or DUBs) are involved in the regulation of central biological processes, including DNA repair, apoptosis, oncogene expression and function, and checkpoint regulation. DUBs are classified in five groups including the ovarian tumor domain-containing DUBs (OTU family), which comprises enzymes that can display linkage-specificity in their recognition and cleavage of diubiquitin moieties. The OTUD6B gene (located on chromosome 8q21.3, 17 kb long) encodes a member of the ovarian tumor domain (OTU)-containing subfamily of deubiquitinating enzymes. OTUD6B shows a widespread expression pattern (The GTEx Portal1), and its primary transcript can be alternatively spliced to produce two main different splicing variants. The first isoform (NM_016023.3, OTUD6B-1) is characterized by seven exons with the start codon in the first one, whereas isoform 2 (NM_001286745.1, OTUD6B-2) includes an additional out-of-frame exon (from here named exon 3) that leads to a premature stop just after three residues. In non-small cell lung cancer, the two main OTUD6B isoforms have been shown to have opposite effects on global protein synthesis and DNA synthesis, with OTUD6B-1 stimulating protein and DNA synthesis and OTUD6B-2 repressing both processes. Biallelic mutations in the OTUD6B gene, were reported to cause an intellectual disability syndrome characterized by seizures and dysmorphic features.

References

Straniero L, et al. (2018) First replication of the involvement of otud6b in intellectual disability syndrome with seizures and dysmorphic features. Front Genet 9 464.Sobol A, et al. (2017) Deubiquitinase otud6b isoforms are important regulators of growth and proliferation. Mol Cancer Res 15 (2): 117-127.Santiago-Sim T, et al. (2017) Biallelic variants in otud6b cause an intellectual disability syndrome associated with seizures and dysmorphic features. Am J Hum Genet 100 (4): 676-688.