Rabbit anti-Human GFAP Polyclonal Antibody | anti-GFAP antibody
Anti-GFAP Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
GFAP; Polyclonal Antibody; Anti-GFAP Antibody; Rabbit Polyclonal; GFAP Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-ALXDRD Antibody; glial fibrillary acidic protein; anti-GFAP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human GFAP
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-GFAP antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:1000-1:10,000
IP: 1-5uL/mg of Lysate
IHC-P: 1:1000-1:10,000
IP: 1-5uL/mg of Lysate
Immunogen
E Coli-derived Human GFAP fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human GFAP fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Western Blot (WB)
(Anti-IVD036 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lane B: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:50 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human GFAP in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(IVD036 was immunoprecipitated using: Lane A:0.5 mg U-251MG Whole Cell Lysate 4 uL anti-IVD036 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-IVD036 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa Observed band size :47 kDa)
Related Product Information for anti-GFAP antibody
Background: GFAP is a cell-specific marker which belongs to the intermediate filament family. It can distinguish astrocytes from other glial cells during development. GFAP is expressed in cells lacking fibronectin. It is a type III intermediate filaments protein which contains three domains: the head, rod and tail domains. GFAP functions in many important entral nervous system (CNS) processes, including cell communication and the functioning of the blood brain barrier. Improper GFAP regulation can cause multiple disorders. Defects in GFAP is related to Alexander disease which is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes.
Product Categories/Family for anti-GFAP antibody
References
Buniatian G, et al., 1998, Biology of the cell. 90(1): 53-61.Chen YS, et al., 2011, Experimental Cell Research. 317(16): 2252-66.Isaacs A, et al., 1998, Genomics. 51(1): 152-4.
