Western Blot (WB)
(TYW3 Antibody)
Rabbit anti-Human, Mouse TYW3 Polyclonal Antibody | anti-TYW3 antibody
TYW3 Antibody
Gene Names
TYW3; C1orf171
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Synonyms
TYW3; Polyclonal Antibody; TYW3 Antibody; C1orf171; tRNA wybutosine-synthesizing protein 3 homolog; tRNA-yW-synthesizing protein 3; tRNA-yW synthesizing protein 3 homolog (S. cerevisiae); anti-TYW3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
TYW3 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW3 are known to exist; this antibody will detect both isoforms.
Form/Format
Liquid; TYW3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
259
Applicable Applications for anti-TYW3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
TYW3 antibody can be used for detection of TYW3 by Western blot at 1 ug/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Conjugate
Unconjugated
Immunogen
TYW3 antibody was raised against a 17 amino acid synthetic peptide near the center of human TYW3.
Then immunogen is located within amino acids 160-210 of TYW3.
Then immunogen is located within amino acids 160-210 of TYW3.
Homology
Predicted species reactivity based on immunogen sequence: Bovine (94%), Pig (94%)
Positive Control
1) Cat No. MBS151686 A549 Cell Lysate.
Preparation and Storage
TYW3 antibody can be stored at 4 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(TYW3 Antibody)
Western Blot (WB)
(TYW3 Antibody)
Related Product Information for anti-TYW3 antibody
TYW3 Antibody: TYW3 is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW3 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
tRNA wybutosine-synthesizing protein 3 homolog isoform 1
NCBI Official Synonym Full Names
tRNA-yW synthesizing protein 3 homolog (S. cerevisiae)
NCBI Official Symbol
TYW3
NCBI Official Synonym Symbols
C1orf171
NCBI Protein Information
tRNA wybutosine-synthesizing protein 3 homolog; tRNA-yW-synthesizing protein 3; tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase
UniProt Protein Name
tRNA wybutosine-synthesizing protein 3 homolog
Protein Family
UniProt Gene Name
TYW3
UniProt Synonym Gene Names
C1orf171; tRNA-yW-synthesizing protein 3
UniProt Entry Name
TYW3_HUMAN
NCBI Description
Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW3 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM, Mar 2008]
Uniprot Description
TYW3: Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Belongs to the TYW3 family.
Protein type: Methyltransferase; EC 2.1.1.282
Chromosomal Location of Human Ortholog: 1p31.1
Molecular Function: methyltransferase activity
Biological Process: methylation; tRNA processing
Similar Products
Product Notes
The TYW3 tyw3 (Catalog #AAA150652) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TYW3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TYW3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). TYW3 antibody can be used for detection of TYW3 by Western blot at 1 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the TYW3 tyw3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TYW3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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