Cadherin 8 Recombinant Protein | CDH8 recombinant protein

Cadherin 8 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120023

• Gene Names: CDH8; Nbla04261
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Cadherin 8; Cadherin 8 Protein; Human; Recombinant (His Tag); Human Cadherin-8/CDH8 Protein (His Tag); Nbla04261 Protein; cadherin 8; type 2; CDH8 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Met621

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 30
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of human CDH8 (NP_001787.2)(Met 1-Met 621) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CDH8 recombinant protein

Background: Cadherins are integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Type I cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of five subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Cadherin 8, also known as CDH 8, is a type II classical cadherin belonging to the cadherin superfamily. As mainly expressed in brain, CDH8 is found in certain nerve cell lines, such as retinoblasts, glioma cells and neuroblasts, and is putatively involved in synaptic adhesion, axon outgrowth and guidance. Human Cadherin 8 is a 799 amino acid single-pass type Itransmembrane protein with a putative 29 aa signal sequence, and a 32 aa propeptide, a 56 aa mature extracellular domain, a 21 aa transmembrane domain and a 157 aa cytoplasmic domain. The human, mouse and rat proteins share approximately 98% homology.

Product Categories/Family for CDH8 recombinant protein

Canonical Wnt Pathway

References

Tanihara, H. et al., 1994, Cell Adhes. Comm. 2:15-26. Kido, M. et al., 1998, Genomics 48:186-194. Yamagata, K. et al., 1999, J. Biol. Chem. 274:19473-19479. Nollet, F. et al., 2000, J. Mol. Biol. 299:551-572. Blaschke, S. et al., 2002, Int. J. Cancer. 101 (4): 327-334. Strausberg,R.L. et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-16903.

NCBI and Uniprot Product Information

• NCBI GI #: 16306539
• NCBI GeneID: 1006
• NCBI Accession #: NP_001787.2
• NCBI GenBank Nucleotide #: NM_001796.4
• UniProt Accession #: P55286; Q14DC6; Q9ULB2; B3KWC1
• Molecular Weight: 88,253 Da
• NCBI Official Full Name: cadherin-8 preproprotein
• NCBI Official Synonym Full Names: cadherin 8, type 2
• NCBI Official Symbol: CDH8
• NCBI Official Synonym Symbols: Nbla04261
• NCBI Protein Information: cadherin-8
• UniProt Protein Name: Cadherin-8
• Protein Family: Cadherin
• UniProt Gene Name: CDH8
• UniProt Entry Name: CADH8_HUMAN

NCBI Description

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed in brain and is putatively involved in synaptic adhesion, axon outgrowth and guidance. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH8: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: plasma membrane; integral to membrane; nerve terminal

Molecular Function: calcium ion binding

Biological Process: intercellular junction assembly and maintenance; synaptic transmission, glutamatergic; response to cold; cell adhesion; homophilic cell adhesion