Rabbit TYMS Polyclonal Antibody | anti-TYMS antibody

TYMS Polyclonal Antibody

Cat. Num. AAA3013625

• Gene Names: TYMS; TS; TMS; HST422
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: TYMS; Polyclonal Antibody; TYMS Polyclonal Antibody; TS; TSase; OK/SW-cl.29; d TMP synthase; EC 2.1.1.45; HsT422; MGC88736; OTTHUMP00000162195; Thymidylate synthase; Thymidylate synthetase; TMS; Tyms; TYMS protein; Tyms thymidylate synthetase; TYSY; anti-TYMS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TYMS polyclonal antibody detects endogenous levels of TYMS protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 313

• Applicable Applications for anti-TYMS antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2,000; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Thymidylate Synthase on different cell lysate using anti-Thymidylate Synthase antibody at 1/1,000 dilution. Positive control: Lane1: Hela Lane2: Jurkat Lane3: Raji Lane4: Mouse testes Lane5: Mouse brain Lane6: Mouse spleen)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Thymidylate Synthase antibody. Counter stained with hematoxylin.)

Related Product Information for anti-TYMS antibody

Thymidylate synthase (TS), also designated TYMS, TMS, TSase and HsT422, uses 5, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor in the synthesis of 2'-deoxythymidine-5'-monophosphate (dTMP), an essential precursor for DNA biosynthesis. TS is an RNA-binding protein that can interact with its own mRNA. The TS/mRNA ribonucleoprotein complex can also associate with a number of other cellular mRNAs, including those corresponding to the p53 tumor suppressor gene and the Myc family of transcription factors. Inhibition of DNA replication and cell death resulting from thymidine depletion occurs when TS enzyme activity is inhibited with substrate or cofactor analogs, making the TS enzyme an important target for chemotherapy. Cancer cells are sensitive to thymidine depletion, as they multiply rapidly.

NCBI and Uniprot Product Information

• NCBI GI #: 136611
• NCBI GeneID: 7298
• UniProt Accession #: P04818; P07607; P45352
• Molecular Weight: 36kDa
• NCBI Official Full Name: Thymidylate synthase
• NCBI Official Synonym Full Names: thymidylate synthetase
• NCBI Official Symbol: TYMS
• NCBI Official Synonym Symbols: TS; TMS; HST422
• NCBI Protein Information: thymidylate synthase
• UniProt Protein Name: Thymidylate synthase
• Protein Family: TYMS opposite strand protein
• UniProt Gene Name: TYMS
• UniProt Synonym Gene Names: TS; TS; TSase
• UniProt Entry Name: TYSY_HUMAN

NCBI Description

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]

Uniprot Description

TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer.

Protein type: Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - one carbon pool by folate; DNA repair, damage; EC 2.1.1.45; Mitochondrial; Methyltransferase

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; nucleolus; nucleus; cytosol

Molecular Function: mRNA binding; protein homodimerization activity; nucleotide binding; drug binding; cofactor binding; thymidylate synthase activity; folic acid binding

Biological Process: immortalization of host cell by virus; methylation; tetrahydrofolate metabolic process; circadian rhythm; uracil metabolic process; developmental growth; response to organophosphorus; response to toxin; response to glucocorticoid stimulus; dTTP biosynthetic process; response to vitamin A; pyrimidine base metabolic process; response to folic acid; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; aging; response to drug; organ regeneration; nucleobase, nucleoside and nucleotide metabolic process; dTMP biosynthetic process; deoxyribonucleoside monophosphate biosynthetic process; G1/S-specific transcription in mitotic cell cycle; response to ethanol; response to cytokine stimulus; cartilage development; pyrimidine nucleoside biosynthetic process; dUMP metabolic process; mitotic cell cycle; response to progesterone stimulus; G1/S transition of mitotic cell cycle