Western Blot (WB)
(Anti-TYMS rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HL60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa(We are unsure as to the identity of these extra bands.))
Rabbit Thymidylate Synthase/TYMS Polyclonal Antibody | anti-TYMS antibody
Anti-Thymidylate Synthase/TYMS Antibody, Rabbit Polyclonal
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
IP: 4-8uL/mg of lysate
Western Blot (WB)
(Anti-TYMS rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HL60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-TYMS rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HL60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa(We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(TYMS was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 uL anti-TYMS rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TYMS rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDa)
Immunoprecipitation (IP)
(TYMS was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 uL anti-TYMS rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TYMS rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDa)
NCBI and Uniprot Product Information
Observed MW: 36kDa
NCBI Description
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008]
Uniprot Description
TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer.
Protein type: Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - one carbon pool by folate; DNA repair, damage; EC 2.1.1.45; Mitochondrial; Methyltransferase
Chromosomal Location of Human Ortholog: 18p11.32
Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; nucleolus; nucleus; cytosol
Molecular Function: mRNA binding; protein homodimerization activity; nucleotide binding; drug binding; cofactor binding; thymidylate synthase activity; folic acid binding
Biological Process: immortalization of host cell by virus; methylation; tetrahydrofolate metabolic process; circadian rhythm; uracil metabolic process; developmental growth; response to organophosphorus; response to toxin; response to glucocorticoid stimulus; dTTP biosynthetic process; response to vitamin A; pyrimidine base metabolic process; response to folic acid; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; aging; response to drug; organ regeneration; nucleobase, nucleoside and nucleotide metabolic process; dTMP biosynthetic process; deoxyribonucleoside monophosphate biosynthetic process; G1/S-specific transcription in mitotic cell cycle; response to ethanol; response to cytokine stimulus; cartilage development; pyrimidine nucleoside biosynthetic process; dUMP metabolic process; mitotic cell cycle; response to progesterone stimulus; G1/S transition of mitotic cell cycle
Research Articles on TYMS
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Product Notes
The TYMS tyms (Catalog #AAA8101835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Thymidylate Synthase/TYMS Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's Thymidylate Synthase/TYMS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 4-8uL/mg of lysate. Researchers should empirically determine the suitability of the TYMS tyms for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thymidylate Synthase/TYMS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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