Rabbit TXK Polyclonal Antibody | anti-TXK antibody

TXK Polyclonal Antibody

Cat. Num. AAA3007396

• Gene Names: TXK; RLK; TKL; BTKL; PTK4; PSCTK5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TXK; Polyclonal Antibody; TXK Polyclonal Antibody; Tyrosine-protein kinase TXK; Protein-tyrosine kinase 4; Resting lymphocyte kinase; PTK4; RLK; anti-TXK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TXK polyclonal antibody detects endogenous levels of TXK protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 527

• Applicable Applications for anti-TXK antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 321-366 of Human TXK.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TXK polyclonal antibody at 1:500 dilutionLane1:Jurkat whole cell lysate (40ug) Lane2:The Spleen tissue lysate of Rat (30ug) Lane3:The Spleen tissue lysate of Mouse (30ug) Lane4:THP-1 whole cell lysate (40ug))

Related Product Information for anti-TXK antibody

The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Emt (also known as Itk or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. Four alternatively spliced forms of Tec are found to be expressed broadly in cells of hematopoietic lineage and hepatocytes. The Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Brutons disease. The Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated.

NCBI and Uniprot Product Information

• NCBI GI #: 148596974
• NCBI GeneID: 7294
• NCBI Accession #: NP_003319.2
• NCBI GenBank Nucleotide #: NP_003319.2
• UniProt Accession #: P42681
• Molecular Weight: ~ 61kDa
• NCBI Official Full Name: tyrosine-protein kinase TXK
• NCBI Official Synonym Full Names: TXK tyrosine kinase
• NCBI Official Symbol: TXK
• NCBI Official Synonym Symbols: RLK; TKL; BTKL; PTK4; PSCTK5
• NCBI Protein Information: tyrosine-protein kinase TXK
• UniProt Protein Name: Tyrosine-protein kinase TXK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: TXK
• UniProt Synonym Gene Names: PTK4; RLK
• UniProt Entry Name: TXK_HUMAN

Uniprot Description

TXK: Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T- cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskekleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell- specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr- 201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor. Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Kinase, protein; TK group; Tec family

Chromosomal Location of Human Ortholog: 4p12

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; nucleus

Molecular Function: protein binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: adaptive immune response; transcription, DNA-dependent; protein amino acid autophosphorylation; cytokine production; T cell receptor signaling pathway; protein amino acid phosphorylation; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; interleukin-4 production; B cell receptor signaling pathway; positive regulation of interferon-gamma production; phospholipase C activation; interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; NK T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation