SerpinD1 active protein

SerpinD1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122543

• Purity: >98% as determined by SDS-PAGE
• Synonyms: SerpinD1; SerpinD1 Protein; Mouse; Recombinant (His Tag); Mouse SerpinD1/HCII Protein (His Tag); AA985900 Protein; AI30344 6 Protein; AI303446 Protein; Hcf2 Protein; HCII Protein; MGC107662 Protein; Serpind1 Protein; serpin peptidase inhibitor; clade D (heparin cofactor); member 1; SerpinD1 active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM HEPES, 150mM NaCl, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser478

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse SerpinD1 precursor (NP_032249.3)(Met1-Ser 478) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to inhibit thombin (Sigma, T4648) cleavage of fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is <1.2nM.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SerpinD1 active protein

Background: SerpinD1, also known as heparin cofactor II, is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

References

Rau JC, et al. (2009) Heparin cofactor II in atherosclerotic lesions from the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) study. Exp Mol Pathol. 87(3): 178-83.Aihara K, et al. (2009) Heparin cofactor II as a novel vascular protective factor against atherosclerosis. J Atheroscler Thromb. 16(5): 523-31.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant mouse SerpinD1 consists of 466 amino acids and predicts a molecular mass of 53.5kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rmSerpinD1 is approximately 65-70kDa as a result of glycosylation.
• Protein Family: Heparin cofactor