Chemiluminescence
(Representative blot from a previous lot. Acid extracts from untreated (lane 1) and sodium butyrate treated (lane 2) HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with antiacetyl-Histone H3 (Lys9), (1:5000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MBS611277 H3 (Lys9), (~17kD).)
Rabbit anti-Human Histone H3, acetylated Polyclonal Antibody | anti-H3 antibody
Histone H3, acetylated (Lys9)
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
SerumSerum
Synonyms
Histone H3; acetylated; Polyclonal Antibody; acetylated (Lys9); Anti -Histone H3; anti-H3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes acetyl-histone H3 (Lys9), Mr 17kD. An additional unknown protein was detected at Mr 23kD. Species Crossreactivity: Yeast and human. Broad species crossreactivity is expected.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid containing 0.05% sodium azide before the addition of glycerol to 30%.
Applicable Applications for anti-H3 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot, Chromatin IP and Beadlyte Histone-Peptide Specificity Assay.
Dilution: Immunoblot Analysis: A 1:5000-1:10,000 dilution of this lot detected acetyl-Histone H3 (Lys9) in acid extracts from sodium butyrate treated HeLa cells.
Chromatin Immunoprecipitation (ChIP): An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from a yeast strain containing a Lysine substitution to Alanine at residue 9.
Beadlyte Histone-Peptide Specificity Assay: A 1:1000-1:5000 dilution of this lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. No crossreactivity with peptides containing acetyl-lysine 14 or acetyl-lysine 27 was observed.
Dilution: Immunoblot Analysis: A 1:5000-1:10,000 dilution of this lot detected acetyl-Histone H3 (Lys9) in acid extracts from sodium butyrate treated HeLa cells.
Chromatin Immunoprecipitation (ChIP): An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from a yeast strain containing a Lysine substitution to Alanine at residue 9.
Beadlyte Histone-Peptide Specificity Assay: A 1:1000-1:5000 dilution of this lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. No crossreactivity with peptides containing acetyl-lysine 14 or acetyl-lysine 27 was observed.
Immunogen
Ovalbumin-conjugated, synthetic peptide (KQTARAcKSTGGK-C) corresponding to aa4-14 of yeast histone H3 acetylated on lysine 9, with a C-terminal cysteine added for conjugation purposes.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chemiluminescence
(Representative blot from a previous lot. Acid extracts from untreated (lane 1) and sodium butyrate treated (lane 2) HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with antiacetyl-Histone H3 (Lys9), (1:5000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MBS611277 H3 (Lys9), (~17kD).)
Chemiluminescence
(Representative blot from a previous lot. Acid extracts from untreated (lane 1) and sodium butyrate treated (lane 2) HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with antiacetyl-Histone H3 (Lys9), (1:5000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MBS611277 H3 (Lys9), (~17kD).)
Immunoprecipitation (IP)
(Chromatin Immunoprecipitation)
Immunoprecipitation (IP)
(Chromatin Immunoprecipitation)
Testing Data
(Beadlyte™ Histone-Peptide Specificity Assay Data:)
Testing Data
(Beadlyte™ Histone-Peptide Specificity Assay Data:)
Product Categories/Family for anti-H3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone H3
NCBI Official Symbol
H3
NCBI Protein Information
histone H3
Protein Family
Research Articles on H3
Similar Products
Product Notes
The H3 (Catalog #AAA611277) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, acetylated (Lys9) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3, acetylated can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot, Chromatin IP and Beadlyte Histone-Peptide Specificity Assay. Dilution: Immunoblot Analysis: A 1:5000-1:10,000 dilution of this lot detected acetyl-Histone H3 (Lys9) in acid extracts from sodium butyrate treated HeLa cells. Chromatin Immunoprecipitation (ChIP): An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from a yeast strain containing a Lysine substitution to Alanine at residue 9. Beadlyte Histone-Peptide Specificity Assay: A 1:1000-1:5000 dilution of this lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. No crossreactivity with peptides containing acetyl-lysine 14 or acetyl-lysine 27 was observed. Researchers should empirically determine the suitability of the H3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, acetylated, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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