Rabbit anti-Human Cannabinoid Receptor I Polyclonal Antibody | anti-CNR1 antibody

Phospho-Cannabinoid Receptor I (Ser316) Rabbit pAb

Cat. Num. AAA4751850

• Gene Names: CNR1; CB1; CNR; CB-R; CB1A; CB1R; CANN6; CB1K5
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity purified
• Synonyms: Cannabinoid Receptor I; Polyclonal Antibody; Phospho-Cannabinoid Receptor I (Ser316) Rabbit pAb; Cannabinoid receptor 1; CB1; CNR; CB-R; CB1A; CB1R; CANN6; CB1K5; anti-CNR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Widely expressed, with highest levels in fetal and adult brain. Expression levels of isoform 2 and isoform 3 are much lower than those of isoform 1.
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-CNR1 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; FC/FACS: 1:50
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser316 of human Cannabinoid Receptor I
• Conjugation: Unconjugated
• Modification: Phosphoralated
• Subcellular Location: Cell membrane, Cell projection, Membrane, Mitochondrion, Mitochondrion outer membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of Phospho-Cannabinoid Receptor I (Ser316) in Hela cell lysates using Phospho-Cannabinoid Receptor I (Ser316) Rabbit pAb(1:1000 diluted). Predicted band size:53kDa. Observed band size:53kDa.)

Related Product Information for anti-CNR1 antibody

This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009]
G-protein coupled receptor for endogenous cannabinoids (eCBs), including N-arachidonoylethanolamide (also called anandamide or AEA) and 2-arachidonoylglycerol (2-AG), as well as phytocannabinoids, such as delta9-tetrahydrocannabinol (THC) (PubMed:15620723, PubMed:27768894, PubMed:27851727). Mediates many cannabinoid-induced effects, acting, among others, on food intake, memory loss, gastrointestinal motility, catalepsy, ambulatory activity, anxiety, chronic pain. Signaling typically involves reduction in cyclic AMP (PubMed:1718258, PubMed:21895628, PubMed:27768894). In the hypothalamus, may have a dual effect on mitochondrial respiration depending upon the agonist dose and possibly upon the cell type. Increases respiration at low doses, while decreases respiration at high doses. At high doses, CNR1 signal transduction involves G-protein alpha-i protein activation and subsequent inhibition of mitochondrial soluble adenylate cyclase, decrease in cyclic AMP concentration, inhibition of protein kinase A (PKA)-dependent phosphorylation of specific subunits of the mitochondrial electron transport system, including NDUFS2. In the hypothalamus, inhibits leptin-induced reactive oxygen species (ROS) formation and mediates cannabinoid-induced increase in SREBF1 and FASN gene expression. In response to cannabinoids, drives the release of orexigenic beta-endorphin, but not that of melanocyte-stimulating hormone alpha/alpha-MSH, from hypothalamic POMC neurons, hence promoting food intake. In the hippocampus, regulates cellular respiration and energy production in response to cannabinoids. Involved in cannabinoid-dependent depolarization-induced suppression of inhibition (DSI), a process in which depolarization of CA1 postsynaptic pyramidal neurons mobilizes eCBs, which retrogradely activate presynaptic CB1 receptors, transiently decreasing GABAergic inhibitory neurotransmission. Also reduces excitatory synaptic transmission. In superior cervical ganglions and cerebral vascular smooth muscle cells, inhibits voltage-gated Ca2+ channels in a constitutive, as well as agonist-dependent manner (PubMed:17895407). In cerebral vascular smooth muscle cells, cannabinoid-induced inhibition of voltage-gated Ca2+ channels leads to vasodilation and decreased vascular tone. Induces leptin production in adipocytes and reduces LRP2-mediated leptin clearance in the kidney, hence participating in hyperleptinemia. In adipose tissue, CNR1 signaling leads to increased expression of SREBF1, ACACA and FASN genes. In the liver, activation by endocannabinoids leads to increased de novo lipogenesis and reduced fatty acid catabolism, associated with increased expression of SREBF1/SREBP-1, GCK, ACACA, ACACB and FASN genes. May also affect de novo cholesterol synthesis and HDL-cholesteryl ether uptake. Peripherally modulates energy metabolism. In high carbohydrate diet-induced obesity, may decrease the expression of mitochondrial dihydrolipoyl dehydrogenase/DLD in striated muscles, as well as that of selected glucose/ pyruvate metabolic enzymes, hence affecting energy expenditure through mitochondrial metabolism. In response to cannabinoid anandamide, elicits a proinflammatory response in macrophages, which involves NLRP3 inflammasome activation and IL1B and IL18 secretion. In macrophages infiltrating pancreatic islets, this process may participate in the progression of type-2 diabetes and associated loss of pancreatic beta-cells (PubMed:23955712).

NCBI and Uniprot Product Information

• NCBI GI #: 237681067
• NCBI GeneID: 1268
• NCBI Accession #: NP_001153698.1
• NCBI GenBank Nucleotide #: NM_001160226.1
• UniProt Accession #: P21554; Q13949; Q495Z0; Q4PLI4; Q4VBM6; Q5JVL5; Q5UB37; Q9UNN0; B2R9T4; E1P512
• Molecular Weight: 52,858 Da
• NCBI Official Full Name: cannabinoid receptor 1 isoform a
• NCBI Official Synonym Full Names: cannabinoid receptor 1 (brain)
• NCBI Official Symbol: CNR1
• NCBI Official Synonym Symbols: CB1; CNR; CB-R; CB1A; CB1R; CANN6; CB1K5
• NCBI Protein Information: cannabinoid receptor 1; central cannabinoid receptor
• UniProt Protein Name: Cannabinoid receptor 1
• Protein Family: Cannabinoid receptor
• UniProt Gene Name: CNR1
• UniProt Synonym Gene Names: CNR; CB-R; CB1
• UniProt Entry Name: CNR1_HUMAN

NCBI Description

This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009]

Uniprot Description

CNR1: Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 6q14-q15

Cellular Component: growth cone; intracellular membrane-bound organelle; integral to plasma membrane; axon; plasma membrane

Molecular Function: cannabinoid receptor activity; drug binding

Biological Process: response to nicotine; maternal process involved in pregnancy; negative regulation of fatty acid beta-oxidation; regulation of synaptic transmission, glutamatergic; positive regulation of apoptosis; response to morphine; positive regulation of acute inflammatory response to antigenic stimulus; response to lipopolysaccharide; sensory perception of pain; glucose homeostasis; negative regulation of blood pressure; negative regulation of dopamine secretion; response to nutrient; aging; negative regulation of mast cell activation; negative regulation of nitric-oxide synthase activity; axonal fasciculation; negative regulation of action potential; response to cocaine; regulation of synaptic transmission, GABAergic; memory; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein signaling, coupled to cyclic nucleotide second messenger; response to ethanol; positive regulation of fever; spermatogenesis; regulation of insulin secretion; positive regulation of blood pressure