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Western Blot (WB) (Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human TUBb Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Human Tubulin Beta Polyclonal Antibody | anti-TUBb antibody

Polyclonal Antibody to Tubulin Beta (TUBb)

Gene Names
TUBB; M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Tubulin Beta; Polyclonal Antibody; Polyclonal Antibody to Tubulin Beta (TUBb); anti-TUBb antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TUBb. It has beenselected for its ability to recognize TUBb in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-TUBb antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
TUBb (Val170~Val419)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Mouse, Rat
Conjugate
No Conjugate
Immunogen
Recombinant TUBb (Val170~Val419) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2060039
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human TUBb Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human TUBb Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded spleen tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded spleen tissue))

Immunohistochemistry (IHC)

(FITC staining on IHC-P Simple: Mcf7 cells))

Immunohistochemistry (IHC) (FITC staining on IHC-P Simple: Mcf7 cells))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,671 Da
NCBI Official Full Name
tubulin beta chain isoform a
NCBI Official Synonym Full Names
tubulin beta class I
NCBI Official Symbol
TUBB
NCBI Official Synonym Symbols
M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
NCBI Protein Information
tubulin beta chain
UniProt Protein Name
Tubulin beta chain
Protein Family
UniProt Gene Name
TUBB
UniProt Synonym Gene Names
TUBB5

NCBI Description

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]

Uniprot Description

TUBB: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO/C1orf88. Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: cell body; cytoplasm; cytoplasmic ribonucleoprotein granule; cytoskeleton; extracellular exosome; extracellular matrix; extracellular region; membrane raft; microtubule; microtubule cytoskeleton; nuclear envelope lumen; nucleus; protein complex

Molecular Function: GTP binding; GTPase activating protein binding; GTPase activity; MHC class I protein binding; protein binding; protein complex binding; protein domain specific binding; structural constituent of cytoskeleton; structural molecule activity; ubiquitin protein ligase binding

Biological Process: cell division; cell motility; cellular process; cytoskeleton-dependent intracellular transport; G2/M transition of mitotic cell cycle; microtubule-based process; natural killer cell mediated cytotoxicity; neutrophil degranulation; regulation of G2/M transition of mitotic cell cycle; spindle assembly

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 6; Skin Creases, Congenital Symmetric Circumferential, 1

Research Articles on TUBb

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Product Notes

The TUBb tubb (Catalog #AAA2028214) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tubulin Beta (TUBb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin Beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the TUBb tubb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin Beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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