Human Phosphoglycerate Mutase 1, Brain (PGAM1) ELISA Kit | PGAM1 elisa kit

Human Phosphoglycerate Mutase 1, Brain (PGAM1) ELISA Kit

Cat. Num. AAA451255

• Gene Names: PGAM1; PGAMA; PGAM-B; HEL-S-35
• Reactivity: Human
• Synonyms: Phosphoglycerate Mutase 1; Brain (PGAM1); Human Phosphoglycerate Mutase 1; Brain (PGAM1) ELISA Kit; PGAMA; PGAM-B; BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PGAM1. No significant cross-reactivity or interference between PGAM1 and analogues was observed.
• Sequence Length: 254

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.61ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PGAM1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PGAM1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGAM1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PGAM1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGAM1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PGAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PGAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PGAM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505753
• NCBI GeneID: 5223
• NCBI Accession #: NP_002620.1
• NCBI GenBank Nucleotide #: NM_002629.3
• UniProt Accession #: P18669; Q9BWC0
• Molecular Weight: 28,804 Da
• NCBI Official Full Name: phosphoglycerate mutase 1 isoform 1
• NCBI Official Synonym Full Names: phosphoglycerate mutase 1
• NCBI Official Symbol: PGAM1
• NCBI Official Synonym Symbols: PGAMA; PGAM-B; HEL-S-35
• NCBI Protein Information: phosphoglycerate mutase 1
• UniProt Protein Name: Phosphoglycerate mutase 1
• Protein Family: Phosphoglycerate mutase
• UniProt Gene Name: PGAM1
• UniProt Synonym Gene Names: PGAMA; PGAM-B

NCBI Description

The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.