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Western Blot (WB)
(Western Blot analysis of Hela cells with A-Raf Polyclonal Antibody.)
Raf Family Antibody Kit | Raf antibody kit
Raf Family Antibody Sampler Kit
Applications
Western Blot
Synonyms
Raf Family; Raf Family Antibody Sampler Kit; Raf antibody kit
Applicable Applications for Raf antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of Hela cells with A-Raf Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of Hela cells with A-Raf Polyclonal Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat kidney tissue using Raf-1 Polyclonal Antibody at dilution of 1:200.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat kidney tissue using Raf-1 Polyclonal Antibody at dilution of 1:200.)
Western Blot (WB)
(Western Blot analysis of COS7 cells with Phospho-Raf-1 (Ser259) Polyclonal Antibody at dilution of 1:2000)
Western Blot (WB)
(Western Blot analysis of COS7 cells with Phospho-Raf-1 (Ser259) Polyclonal Antibody at dilution of 1:2000)
Western Blot (WB)
(Western Blot analysis of Hela cells with Phospho-Raf-1 (Ser338) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of Hela cells with Phospho-Raf-1 (Ser338) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of 293 cells using Phospho-Raf-1 (Ser296) Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of 293 cells using Phospho-Raf-1 (Ser296) Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of 293T cells using Raf-B Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of 293T cells using Raf-B Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of 293T cells with Phospho-Raf-B (Ser446) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of 293T cells with Phospho-Raf-B (Ser446) Polyclonal Antibody at dilution of 1:1000)
Related Product Information for Raf antibody kit
Background: A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP- bound Ras to activate the MEK-MAP kinase pathway. Activation of c- Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499. p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity. Ser338 of c-Raf corresponds to similar sites in A- Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf. Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively. While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed. Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439) and lacks a site equivalent to Tyr341 of c-Raf. Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma. Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301, and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events.
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Product Notes
The Raf (Catalog #AAA2550073) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Raf Family can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Raf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Raf Family, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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