Chemiluminescence
(MBS622661 (0.5ug/ml) staining of Human Skeletal muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat anti-Human TRC8 Polyclonal Antibody | anti-Trc8 antibody
TRC8 (Translocation in renal carcinoma on Chromosome 8, RNF139, ring finger protein 139, HRCA1, MGC31961, RCA1)
Gene Names
Trc8; CG2304; DmelCG2304; Dtrc8; TRC8
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by antigen immunoaffinity chromatography.
Synonyms
TRC8; Polyclonal Antibody; TRC8 (Translocation in renal carcinoma on Chromosome 8; RNF139; ring finger protein 139; HRCA1; MGC31961; RCA1); Anti -TRC8 (Translocation in renal carcinoma on Chromosome 8; anti-Trc8 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human TRC8 at ~150kD. Species sequence homology: canine.
Purity/Purification
Affinity Purified
Purified by antigen immunoaffinity chromatography.
Purified by antigen immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Applicable Applications for anti-Trc8 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:16000
Western Blot: 0.5-1.5ug/ml
Dilution: ELISA: 1:16000
Western Blot: 0.5-1.5ug/ml
Immunogen
Synthetic peptide corresponding to C-HTGAAAEEFNDDT, from the C Terminus of human TRC8 (NP_009149.2).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chemiluminescence
(MBS622661 (0.5ug/ml) staining of Human Skeletal muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
(MBS622661 (0.5ug/ml) staining of Human Skeletal muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-Trc8 antibody
The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.
Product Categories/Family for anti-Trc8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,235 Da
NCBI Official Full Name
Trc8, isoform C
NCBI Official Symbol
Trc8
NCBI Official Synonym Symbols
CG2304; DmelCG2304; Dtrc8; TRC8
NCBI Protein Information
CG2304 gene product from transcript CG2304-RB; Trc8, isoform B; CG2304-PB; Trc8-PB; Trc8, isoform D; CG2304-PD; Trc8-PD; Trc8, isoform A; CG2304-PA; Trc8-PA; Trc8, isoform C; CG2304-PC; Trc8-PC
UniProt Protein Name
Protein TRC8 homolog
Protein Family
UniProt Gene Name
Trc8
UniProt Entry Name
TRC8_DROME
Uniprot Description
Function: Plays a role in growth inhibition that is dependent upon COP9 signalosome subunits CSN5 and CSN6. May modulate signalosome levels or compartmentalization. Probably functions in the same or a related pathway to VHL during early midline development. Ref.1 Ref.5
Subunit structure: Interacts with VHL. Interacts with the MPN domain of CSN5. Interacts with EIF3F and EIF3H. Ref.1 Ref.6
Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1.
Developmental stage: Expressed in oocytes throughout development. Ref.1
Domain: The RING-type zinc finger domain may be essential for ubiquitin ligase activity
By similarity. UniProtKB O75485
Sequence similarities: Contains 1 RING-type zinc finger.
Sequence caution: The sequence AAL39520.1 differs from that shown. Reason: Erroneous initiation.
Similar Products
Product Notes
The Trc8 trc8 (Catalog #AAA622661) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The TRC8 (Translocation in renal carcinoma on Chromosome 8, RNF139, ring finger protein 139, HRCA1, MGC31961, RCA1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRC8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:16000 Western Blot: 0.5-1.5ug/ml. Researchers should empirically determine the suitability of the Trc8 trc8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRC8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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