Western Blot (WB)
(MBS624265 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat anti-Human Mel18 Polyclonal Antibody | anti-zuc antibody
Mel18 (RNF110, MEL-18, Polycomb Group RING Finger Protein 2, DNA-binding Protein Mel-18, RING Finger Protein 110, Zinc Finger Protein 144, PCGF2, ZNF144)
Purified by peptide affinity chromatography.
Purified by peptide affinity chromatography.
Dilution: Peptide ELISA Titer: 1:16,000
Western Blot: 1-2ug/ml detects ~40kD band observed in Human Lung lysates (calculated MW of 37.8kD according to NP_009075.1).
Western Blot (WB)
(MBS624265 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(MBS624265 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Cardiolipin hydrolase present at the mitochondrial outer membrane required for piRNA metabolic process. Acts by catalyzing the hydrolysis of cardiolipin (diphosphatidylglycerol) to form phosphatidate (phosphatidic acid or PA) at the mitochondrial outer membrane surface, promoting the piRNA metabolic process. Plays a key role in primary biogenesis of piRNAs and is required during oogenesis to repress transposable elements and prevent their mobilization. piRNAs mediate the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Involved in trans-silencing effect (TSE), a homology-dependent repression mechanism by which a P-transgene inserted in subtelomeric heterochromatin via its role in piRNA biogenesis. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Subunit structure: Homodimer
By similarity.
Subcellular location: Mitochondrion outer membrane; Single-pass membrane protein. Note: Was initially reported to localize to the meiotic nuage, also named P granule (Ref.6). However, it was later shown that the EGFP tag at N-terminus used in initial experiments interfered with mitochondrial localization (Ref.12 and Ref.9). Ref.9 Ref.12
Domain: In contrast to other members of the phospholipase D family, contains only one PLD phosphodiesterase domain, suggesting that it has a single half-catalytic and requires homodimerization to form a complete active site
By similarity.
Disruption phenotype: Defects in mid oogenesis. Females are viable but produce eggs with a range of dorso-ventral patterning defects. Flies lay few eggs, all of which are completely ventralized and often collapsed. Effects are due to defects in piRNA biogenesis and derepression of retrotransposons. Defects are not only present in germ cells but also in somatic cells of the ovary. Ref.6
Sequence similarities: Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily.Contains 1 PLD phosphodiesterase domain.
Research Articles on zuc
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Product Notes
The zuc zuc (Catalog #AAA624265) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Mel18 (RNF110, MEL-18, Polycomb Group RING Finger Protein 2, DNA-binding Protein Mel-18, RING Finger Protein 110, Zinc Finger Protein 144, PCGF2, ZNF144) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mel18 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Peptide ELISA Titer: 1:16,000 Western Blot: 1-2ug/ml detects ~40kD band observed in Human Lung lysates (calculated MW of 37.8kD according to NP_009075.1). Researchers should empirically determine the suitability of the zuc zuc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mel18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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