Rabbit anti-Human, Mouse TRAP150 Polyclonal Antibody | anti-THRAP3 antibody

TRAP150 (Thyroid Hormone Receptor-associated Protein Complex 150kD Component, TR150, Thyroid Hormone Receptor Associated Protein 3, THRAP3)

Cat. Num. AAA647747

• Gene Names: THRAP3; TRAP150
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TRAP150; Polyclonal Antibody; TRAP150 (Thyroid Hormone Receptor-associated Protein Complex 150kD Component; TR150; Thyroid Hormone Receptor Associated Protein 3; THRAP3); Anti -TRAP150 (Thyroid Hormone Receptor-associated Protein Complex 150kD Component; anti-THRAP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Thyroid hormone receptor associated protein 3. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

• Applicable Applications for anti-THRAP3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.1-1ug/ml; Immunohistochemistry (paraffin): 10ug/ml
• Immunogen: Synthetic peptide corresponding to aa930-955 of human Thyroid hormone receptor associated protein 3.
• Positive Control: HeLa cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of human Thyroid hormone receptor associated protein 3 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using at 0.1ug/ml.)

Immunohistochemistry (IHC)

(IHC analysis of human colon using at 10ug/ml.)

Related Product Information for anti-THRAP3 antibody

THRAP3 is a 955aa novel nuclear receptor and a member of the Thyroid hormone receptor associated proteins with a LISH domain and no obvious known sequence motifs. THRAP3 is a 150kD subunit of the large multiprotein complex TRAP and is important in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It is also known to play an important role in transcriptional coactivation. It is known to interact with DKC1, nop5:nap65 and fibrillarin. From the Northern blot analysis of several human tissues it was found that TRAP150 is ubiquitously expressed.

Product Categories/Family for anti-THRAP3 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 167234419
• NCBI GeneID: 9967
• NCBI Accession #: NP_005110.2
• NCBI GenBank Nucleotide #: NM_005119.3
• UniProt Accession #: Q9Y2W1; Q5VTK6; D3DPS5
• Molecular Weight: 108,666 Da
• NCBI Official Full Name: thyroid hormone receptor-associated protein 3
• NCBI Official Synonym Full Names: thyroid hormone receptor associated protein 3
• NCBI Official Symbol: THRAP3
• NCBI Official Synonym Symbols: TRAP150
• NCBI Protein Information: thyroid hormone receptor-associated protein 3; thyroid hormone receptor-associated protein, 150 kDa subunit; thyroid hormone receptor-associated protein complex 150 kDa component
• UniProt Protein Name: Thyroid hormone receptor-associated protein 3
• Protein Family: Thyroid hormone receptor-associated protein
• UniProt Gene Name: THRAP3
• UniProt Synonym Gene Names: TRAP150; Trap150
• UniProt Entry Name: TR150_HUMAN

Uniprot Description

Function: Involved in pre-mRNA splicing. Remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). Can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. May be involved in nuclear mRNA decay. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. Involved in cyclin-D1/CCND1 mRNA stability probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in response to DNA damage. Is excluced from DNA damage sites in a manner that parallels transcription inhibition; the function may involve the SNARP complex. Initially thought to play a role in transcriptional coactivation through its association with the TRAP complex; however, it is not regarded as a stable Mediator complex subunit. Ref.21 Ref.22 Ref.26

Subunit structure: Associated with the large multiprotein complex TRAP (Mediator complex-like). Interacts with SFPQ; the interaction is dependent on SFPQ phosphorylation at 'Thr-687' and inhibits binding of SFPQ to an ESS1 exonic splicing silencer element-containing RNA. Interacts with NXF1. Component of the SNARP complex which consists at least of SNIP1, SNW1, THRAP3, BCLAF1 and PNN. Associated with spliced mRNP complexes. Ref.1 Ref.10 Ref.12 Ref.21 Ref.22

Subcellular location: Nucleus Ref.22.

Tissue specificity: Ubiquitous. Ref.1

Sequence caution: The sequence AAH37554.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.