Western Blot/Blotting
(Western blot analysis of TLR3 in K562 cell lysate with TLR3 antibody at (A) 0.5, (B) 1 and (C) 2 g/ml.)
Rabbit anti-Human, Mouse Toll-like receptor 3 (TLR3) Polyclonal Antibody | anti-TLR3 antibody
Toll-like receptor 3 (TLR3) (NT)
Western Blot/Blotting
(Western blot analysis of TLR3 in K562 cell lysate with TLR3 antibody at (A) 0.5, (B) 1 and (C) 2 g/ml.)
Western Blot/Blotting
(Western blot analysis of TLR3 in K562 cell lysate with TLR3 antibody at (A) 0.5, (B) 1 and (C) 2 g/ml.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.15
Subunit structure: Monomer and homodimer; dimerization is triggered by ligand-binding and is required for TLR3 signaling. Interacts (via transmembrane domain) with UNC93B1
By similarity. Interacts with TICAM1 (via the TIR domain); mediates TLR3 signaling. Interacts with SRC; upon binding of double-stranded RNA. Ref.7 Ref.8 Ref.9 Ref.10 Ref.13 Ref.15
Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome membrane Ref.10.
Tissue specificity: Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. Ref.2
Domain: ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18
By similarity.
Post-translational modification: Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. Ref.15 Ref.16
Polymorphism: The Phe-412 allele (dbSNP:rs3775291) occurs with a frequency of 30% in populations with European and Asian ancestry, and confers some natural resistance to HIV-1 infection.
Involvement in disease: Herpes simplex encephalitis 2 (HSE2) [MIM:613002]: A rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system. Ref.17
Sequence similarities: Belongs to the Toll-like receptor family.Contains 22 LRR (leucine-rich) repeats.Contains 1 LRRCT domain.Contains 1 LRRNT domain.Contains 1 TIR domain.
Research Articles on TLR3
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Product Notes
The TLR3 tlr3 (Catalog #AAA395777) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Toll-like receptor 3 (TLR3) (NT) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Toll-like receptor 3 (TLR3) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the TLR3 tlr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Toll-like receptor 3 (TLR3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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