Rabbit anti-Human, Mouse Toll-like receptor 5 (TLR5) Polyclonal Antibody | anti-TLR5 antibody

Toll-like receptor 5 (TLR5) (CT)

Cat. Num. AAA395840

• Gene Names: TLR5; TIL3; SLEB1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen Immunoaffiinity Purification
• Synonyms: Toll-like receptor 5 (TLR5); Polyclonal Antibody; Toll-like receptor 5 (TLR5) (CT); TLR5 (CT): Toll-like receptor 5; anti-TLR5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Immunoaffiinity Purification
• Form/Format: Provided as solution in phosphate buffered saline with 0.02% sodium azide
• Sequence Length: 858

• Applicable Applications for anti-TLR5 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: Rabbit polyclonal TLR5 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human TLR5.
• Positive Control: Found in the membrane, TLR5 is hghly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.6
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Immunohistochemistry (IHC)

(Western blot (left) analysis of TLR5 in THP-1 cell lysate with TLR5 antibody at (A) 0.5, (B) 1 and (C) 2 g/ml.)

Related Product Information for anti-TLR5 antibody

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2). The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (3) so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria and will cause the activation of NF- B, leading to the activation of TNF-alpha; and other cytokines (4). A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires? disease and demonstrates the importance of TLR5 in the innate immune response (5).

Product Categories/Family for anti-TLR5 antibody

Polyclonal Antibody; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 16751843
• NCBI GeneID: 7100
• NCBI Accession #: NP_003259.2
• NCBI GenBank Nucleotide #: NM_003268.5
• UniProt Accession #: O60602; O15456; Q32MI3; B1AZ05; B3Y633; D3DTB8
• Molecular Weight: 97.8 kD
• NCBI Official Full Name: toll-like receptor 5
• NCBI Official Synonym Full Names: toll-like receptor 5
• NCBI Official Symbol: TLR5
• NCBI Official Synonym Symbols: TIL3; SLEB1
• NCBI Protein Information: toll-like receptor 5; toll/interleukin-1 receptor-like protein 3
• UniProt Protein Name: Toll-like receptor 5
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR5
• UniProt Synonym Gene Names: TIL3
• UniProt Entry Name: TLR5_HUMAN

NCBI Description

This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009]

Uniprot Description

Function: Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Ref.10

Subunit structure: Binds MYD88 via their respective TIR domains

By similarity. Homodimer both in the absence and presence of ligand. Ref.14

Subcellular location: Membrane; Single-pass type I membrane protein

By similarity.

Tissue specificity: Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.

Post-translational modification: Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines. Ref.12 Ref.13Phosphorylated at Tyr-798 upon flagellin binding; required for signaling. Ref.12 Ref.13

Polymorphism: Individuals with a common stop codon polymorphism in position 392 are unable to mediate flagellin signaling. This polymorphism acts in a dominant fashion and is associated with susceptibility to pneumonia caused by Legionella pneumophila [

MIM:608556]. It also provides protection against systemic lupus erythematosus.

Involvement in disease: Systemic lupus erythematosus 1 (SLEB1) [MIM:601744]: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.11

Sequence similarities: Belongs to the Toll-like receptor family.Contains 15 LRR (leucine-rich) repeats.Contains 1 TIR domain.