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Western Blot (WB) (Anti-TNIP1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: U251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:72 kDa (We are unsure as to the identity of these extra bands.))

Rabbit anti-Human TNIP1 Polyclonal Antibody | anti-TNIP1 antibody

Anti-TNIP1 Antibody, Rabbit Polyclonal

Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
TNIP1; Polyclonal Antibody; Anti-TNIP1 Antibody; Rabbit Polyclonal; TNIP1 Antibody; Rabbit PAb; Antigen Affinity Purified; TNFAIP3 interacting protein 1; Anti-ABIN-1 Antibody; Anti-NAF1 Antibody; Anti-nip40-1 Antibody; Anti-VAN Antibody; anti-TNIP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TNIP1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TNIP1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human TNIP1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human TNIP1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-TNIP1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: U251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:72 kDa (We are unsure as to the identity of these extra bands.))

Western Blot (WB) (Anti-TNIP1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: U251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:72 kDa (We are unsure as to the identity of these extra bands.))

Immunohistochemistry (IHC)

(Immunochemical staining of human TNIP1 in human skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC) (Immunochemical staining of human TNIP1 in human skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-TNIP1 antibody
TNFalpha-induced protein 3-interacting protein 1 (TNIP1) protein is increasingly being recognized as a key repressor of inflammatory signaling and a potential factor in multiple autoimmune diseases. TNIP1 represses signaling pathways initiated by specific nuclear and transmembrane receptors. This effect results in reduced activity of distinct transcription factors such as retinoic acid receptors (RAR), peroxisome-proliferator-activated receptors (PPAR), and NFkappaB. TNIP1 is a widely expressed ubiquitin sensor that binds to the ubiquitin-editing protein A20 and restricts TNF-and TLR-induced signals. TNIP1-null and TNIP1-knockin defective for ubiquitin-binding mice show increased liver apoptosis, and enlarged spleen and lymph nodes, respectively. According to genome-wide association studies (GWAS), the TNIP1 gene is strongly linked to the susceptibility of psoriasis.
References
Shamilov R, et al. (2018) Tnip1 in autoimmune diseases: Regulation of toll-like receptor signaling. J Immunol Res 2018 3491269.Ramirez VP, et al. (2015) Tnip1 reduction of hspa6 gene expression occurs in promoter regions lacking binding sites for known tnip1-repressed transcription factors. Gene 555 (2): 430-437.Kawasaki A, et al. (2010) Association of tnfaip3 interacting protein 1, tnip1 with systemic lupus erythematosus in a japanese population: A case-control association study. Arthritis Res Ther 12 (5): R174.Chen Y, et al. (2015) Downregulation of tnip1 expression leads to increased proliferation of human keratinocytes and severer psoriasis-like conditions in an imiquimod-induced mouse model of dermatitis. PLoS One 10 (6): e0127957.

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Product Notes

The TNIP1 (Catalog #AAA8108415) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TNIP1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TNIP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the TNIP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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